CHITINASE, BETA-1,3-GLUCANASE, OSMOTIN, AND EXTENSIN ARE EXPRESSED IN TOBACCO EXPLANTS DURING FLOWER FORMATION
CHITINASE, BETA-1,3-GLUCANASE, OSMOTIN, AND EXTENSIN ARE EXPRESSED IN TOBACCO EXPLANTS DURING FLOWER FORMATION
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DOI：
10.1105/tpc.2.7.673
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发表时间：
1990-07-01
期刊：
影响因子：
11.6
通讯作者：
DENNIS, ES
中科院分区：
文献类型：
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作者：
NEALE, AD;WAHLEITHNER, JA;DENNIS, ES
从启动花发育的烟草薄细胞层外植体中分离的五个基因家族的序列分析[Meeks-Wagner et al.（1989）. Plant Cell 1，25-35]显示两个编码病程相关蛋白碱性几丁质酶和碱性β-几丁质酶。1，3-葡聚糖酶，而第三个编码细胞壁蛋白延伸素，这也积累在病原体攻击。另一个序列家族编码水胁迫诱导的蛋白质Aparthotin [Singh等人（1989）。Plant Physiol.90，1096-1101]。我们发现，病毒感染和创伤也能诱导表达，因此，可以认为是一种与病程相关的蛋白。这些基因在从头花形成过程中在外植体中高度表达，但在形成营养芽的外植体中不表达[Meeks-Wagner et al.（1989）. Plant Cell 1，25-35]，在日间中性和光响应烟草植物中也受到发育调节，在根中具有高表达水平，在包括花的其他植物器官中具有中至低水平表达。一个未鉴定的基因家族FB 7 -4在基部节间中表达水平最高。我们的研究结果表明，这些基因，其中一些传统上被认为是编码病原体相关蛋白，也有一个复杂的协会与正常的发育过程，包括花的反应，在健康的植物。
Sequence analysis of five gene families that were isolated from tobacco thin cell layer explants initiating floral development [Meeks-Wagner et al. (1989). Plant Cell 1, 25-35] showed that two encode the pathogenesis-related proteins basic chitinase and basic .beta.-1,3-glucanase, while a third encodes the cell wall protein extensin, which also accumulates during pathogen attack. Another sequence family encodes the water stress-induced protein osmotin [Singh et al. (1989). Plant Physiol. 90, 1096-1101]. We found that osmotin was also induced by viral infection and wounding, hence, could be considered a pathogenesis-related protein. These genes, which were highly expressed in explants during de novo flower formation but not in explants forming vegetative shoots [Meeks-Wagner et al. (1989). Plant Cell 1, 25-35], were also regulated developmentally in day-neutral and photoresponsive tobacco plants with high expression levels in the roots and moderate- to low-level expression in other plant organs including flowers. An unidentified gene family, FB7-4, had its highest level of expression in the basal internodes. Our findings indicate that these genes, some of which are conventionally considered to encode pathogen-related proteins, also have a complex association with normal developmental processes, including the floral response, in healthy plants.