Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells
Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells
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DOI：
10.1007/bf02890379
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Gerdes;H. Stein;D. Y. Mason;Andreas Ziegler
中科院分区：
文献类型：
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作者：
J. Gerdes;H. Stein;D. Y. Mason;Andreas Ziegler
人淋巴组织的b依赖区含有被称为树突状网状细胞（DRC）的非淋巴、非吞噬细胞。这些细胞只能在常规染色的组织学切片中偶尔检测到。最近，我们通过制备一种单克隆抗体r4 /23克服了这一限制，该单克隆抗体可以选择性地与DRC反应。因此，通过将优化的免疫过氧化物酶方法应用于冷冻切片，可以原位检测DRC。为了确定DRC的抗原谱，用r4 /23和大量其他单克隆抗体或常规抗血清对人扁桃体的一系列冷冻切片进行免疫染色。此外，用这些试剂对扁桃体的触摸印迹和DRC浓度增加的细胞悬液的细胞离心玻片进行免疫染色。DRC被证明对μ、γ、α、κ和λ链、补体成分C3b、C3b受体、C3d受体、HLA-A、B、C抗原、人类ia样抗原、常见ALL抗原（cALLa）和单核/巨噬细胞谱系特征抗原呈阳性。DRC不表达δ链、T细胞抗原或在交错网状细胞（IDC）和朗格汉斯细胞上表达的抗原。从增生性扁桃体制备的触觉印迹和悬浮液中发现DRC是具有10个或更多细胞核的巨细胞。在某些滤泡增生和中心细胞-中心细胞淋巴瘤的病例中，有几个核的DRC也可在原位检测到。这些结果表明：(1)DRC的表型与淋巴组织中所有其他细胞类型的表型不同；(2)DRC的表型与单核/巨噬细胞系列细胞的表型最接近，从而表明DRC与这些细胞系有关；(3)DRC是多核巨细胞。
The B-dependent areas of human lymphoid tissue contain non-lymphoid, non-phagocytic cells known as dendritic reticulum cells (DRC). These cells can be detected only very occasionally in routinely stained histologic sections. Recently we were able to overcome this limitation by preparing a monoclonal antibody, termed R 4/23, that reacts selectively with DRC. Thus by using an optimized immunoperoxidase method applied to frozen sections, it is possible to detect DRC in situ. To determine the antigenic profile of DRC, serial frozen sections of human tonsils were immunostained with R 4/23 and a large panel of other monoclonal antibodies or conventional antisera. In addition, touch imprints of tonsils and cytocentrifuge slides of cell suspensions with increased concentrations of DRC were immunostained with these reagents. DRC proved to be positive for μ, γ, α, κ and λ chains, complement component C3b, C3b receptors, C3d receptors, HLA-A,B,C antigens, human Ia-like antigens, common ALL antigen (cALLa), and antigens that are characteristic of the monocyte/macrophage lineages. DRC did not express δ chains, T cell antigens, or antigens that are expressed on interdigitating reticulum cells (IDC) and Langerhans cells. DRC in touch imprints and suspensions prepared from hyperplastic tonsils were found to be giant cells often with 10 or more nuclei. In certain cases of follicular hyperplasia and of centroblastic-centrocytic lymphoma, DRC with several nuclei were also detectable in situ.These results show that (1) the phenotype of DRC differs from that of all other cell types in lymphoid tissue, (2) this phenotype most nearly resembles that of cells of the monocyte/macrophage series, thus suggesting that DRC are related to these cell lineages, and (3) DRC are multinucleated giant cells.