AUDITORY BRAIN-STEM RESPONSES AFTER OVARIECTOMY AND ESTROGEN REPLACEMENT IN RAT
AUDITORY BRAIN-STEM RESPONSES AFTER OVARIECTOMY AND ESTROGEN REPLACEMENT IN RAT
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DOI：
10.1016/0378-5955(94)90112-0
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发表时间：
1994-11-01
期刊：
影响因子：
2.8
通讯作者：
MOYER, J
中科院分区：
文献类型：
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作者：
COLEMAN, JR;CAMPBELL, D;MOYER, J
以前的工作表明，卵巢激素对听觉系统诱发电位的可能影响。本课题旨在研究卵巢切除后给予雌激素替代治疗对听性脑干反应和中潜伏期反应的影响。将90只日龄Long-Evans hooded大鼠麻醉进行双侧卵巢切除术（上），并在3周后进行记录。在记录前一周，一些卵巢切除组接受10、100或500 μ g/kg倍美力花生油皮下注射，其他未手术动物接受溶媒注射。在Rompun/Ketamine下，使用针电极和纯音刺激参数从头顶/下巴进行记录。使用40 kHz音调刺激的结果显示，对于波1a、1an、1b、11、111、111 n和1V/V，以上动物的平均潜伏期长于100 μ g/kg倍美力组动物。11和111。与仅过度动物相比，10岁以上组的波1b、1bn、11和111出现潜伏期缩短，但500岁以上组仅在波11出现潜伏期缩短。来自8 kHz刺激的数据也表明，在波1bn和Vn处，超过100组和超过100组之间存在显著差异。峰间潜伏期的差异，特别是波1a和11之间的观察，表明中央以及耳蜗参与激素的作用。中潜伏期反应的检查显示，在40 kHz刺激下，中潜伏期反应的第三个组成部分，卵巢切除动物的潜伏期显著延长的受试者之间的潜伏期和振幅的变化相当大。这些结果表明，卵巢切除术后听觉信息的神经生理学处理的变化被逆转雌激素治疗，这可能会改变外周和中枢听觉结构的活动。因此，听觉系统处理的改变为绝经期手术模型的研究提供了依赖性措施。
Previous work has suggested possible influences of ovarian hormones on evoked potentials in the auditory system. The aim of this project was to study the effects of ovariectomy and subsequent administration of estrogen replacement on the auditory brainstem response and the middle latency response. Groups of 90 day-old Long-Evans hooded rats were anesthetized for bilateral ovariectomies (over) and recordings made 3 weeks later. During the week prior to recordings some ovariectomized groups received subcutaneous injections of 10, 100 or 500 mu g/kg Premarin in peanut oil, and other unoperated animals received vehicle injections. Recordings from vertex/chin using needle electrodes and pure tone stimulus parameters were made under Rompun/Ketamine. The results using 40 kHz tone stimuli showed that mean latencies for over animals were longer than animals in the 100 mu g/kg Premarin group for waves 1a, 1an, 1b, 11, 111, 111n, and 1V/V. Other posthoc comparisons at 40 kHz stimulation revealed differences between control and 100 mu g/kg Premarin groups for latencies of waves 1b, 1bn, 11 and 111. Latency reduction appeared for waves 1b, 1bn, 11 and 111 for the 10 over group, but only at wave 11 for the 500 over group, compared to over-only animals. Data from 8 kHz stimulation also demonstrated significant differences between the over and over 100 groups at waves 1bn and Vn. Observations of interpeak latency differences, especially between waves 1a and 11, suggested central as well as cochlear involvement in hormone action. Examination of the middle-latency responses revealed considerable variability of latencies and amplitudes among subjects with latencies of ovariectomy animals significantly lengthened for the third component of the middle latency response at 40 kHz stimulation. These results suggest that changes in neurophysiological processing of auditory information followed by ovariectomy are reversed by estrogen treatments which may alter activity in peripheral and central auditory structures. Modification of processing in the auditory system thus provides dependent measures for study in the surgical model of menopause.