Localization of prolyl endopeptidase mRNA in small growing follicles of porcine ovary
Localization of prolyl endopeptidase mRNA in small growing follicles of porcine ovary
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DOI：
10.1002/(sici)1098-2795(199806)50:2
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发表时间：
1998-06
影响因子：
2.5
通讯作者：
A. Kimura;J. Ohnishi;Hikari Okimura;T. Hamabata;Takayuki Takahashi
中科院分区：
文献类型：
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作者：
A. Kimura;J. Ohnishi;Hikari Okimura;T. Hamabata;Takayuki Takahashi
脯氨酰内肽酶 (EC3.4.21.26) 被认为是一种独特的胞内酶，可催化 Pro-X 的内部肽键水解。本研究调查了猪卵巢卵泡中脯氨酰内肽酶活性及其mRNA的分布。来自小卵泡的卵泡液和颗粒细胞部分都比来自大卵泡的卵泡液和颗粒细胞部分表现出更高的活性。分子克隆和Northern印迹分析表明，卵巢中仅表达一种脯氨酰内肽酶基因。此外，原位杂交研究表明，小卵泡颗粒细胞层中脯氨酰内肽酶 mRNA 表达比大卵泡的颗粒细胞层更明显，表明其在卵泡发育早期的重要性。摩尔。重现。开发。 50:121–127, 1998。© 1998 Wiley-Liss, Inc.
Prolyl endopeptidase (EC3.4.21.26) has been considered a unique intracellular enzyme catalyzing internal peptide bond hydrolysis of Pro‐X. In this study, the distribution of prolyl endopeptidase activity and its mRNA was investigated in the follicles of porcine ovary. Both follicular fluid and granulosa cell fractions from small follicles showed higher activity than those from large follicles. Molecular cloning and Northern blot analysis suggested that only one species of prolyl endopeptidase gene was expressed in the ovary. In addition, in situ hybridization study revealed that the prolyl endopeptidase mRNA expresssion was more noticeable in the granulosa cell layers of small ovarian follicles than in those of large follicles, suggesting its importance in the early stage of follicular development. Mol. Reprod. Dev. 50:121–127, 1998. © 1998 Wiley‐Liss, Inc.