The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood.
The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood.
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从正常人血液中获得的“单核”细胞组分中单核细胞的百分比。
DOI：
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发表时间：
1974
影响因子：
4.4
通讯作者：
Dorothea Zucker
中科院分区：
文献类型：
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作者：
Dorothea Zucker
通过Ficoll-Hypaque离心获得的人外周血"单核"细胞组分含有44%的能够吞噬乳胶颗粒的细胞。这些细胞可以在与铁屑一起孵育后通过磁力去除。玻璃棉柱过滤效果较差。在从CLL患者获得的细胞中发现非常少的吞噬细胞。它的结论是，单核细胞在正常血液中的百分比是至少两倍高，是公认的常规涂片。这些观察结果表明，当"单核"细胞用于与淋巴细胞功能有关的研究时，需要进一步的纯化步骤。
Human peripheral blood “mononuclear” cell fractions obtained by Ficoll-Hypaque centrifugation contained 44% of cells able to phagocytose latex particles. Such cells could be removed magnetically following their incubation with iron filings. Glass wool column filtration was less effective. Very few phagocytic cells were found among cells obtained from patients with CLL. It is concluded that the percentage of monocytes in normal blood is at least twice as high as is commonly recognized on routine smears. These observations point up the need for further purification steps whenever “mononuclear” cells are used in studies concerned with the function of lymphocytes.