A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model.
A simple rat model of in situ reversible obstructive jaundice in situ reversible obstructive jaundice model.
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DOI：
10.4174/astr.2017.92.6.389
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发表时间：
2017-06
影响因子：
1.4
通讯作者：
Dong JH
中科院分区：
文献类型：
--
作者：
Huang X;Li CH;Zhang AQ;Kong Z;Gu WQ;Dong JH
目的建立一种简便、可靠、低发病率、低死亡率的大鼠原位可逆性梗阻性黄疸模型。将大鼠分为4组，每组8只：假手术组(SH)仅行开腹手术，对照组(ID-C)行胆总管十二指肠吻合术，新内引流组(ID-N)和长期内引流组(ID-L)行胆总管十二指肠吻合术。所有引流组术前7d行胆总管结扎术。除ID-L组大鼠在处死前28d存活外，其余大鼠于末次手术后7d处死取材。评估体重、肝功能、组织病理学改变、发病率和死亡率。ID-C组1只大鼠死亡，2只大鼠出现输卵管阻塞并发症。ID-N组和ID-L组无死亡和并发症。长期观察ID-L组引流管保持通畅。引流7d后，ID-C组和ID-N组的体重无明显差异。引流7d后，ID-C组和ID-N组肝功能未完全恢复，但仅ID-C组与SH组和ID-L组比较差异有统计学意义。ID-C组门脉周围炎症和胆管增生较ID-N组严重。本研究提供了一种高效、简便、可靠的大鼠模型，特别适用于可逆性梗阻性黄疸的长期或连续研究。
To develop a simple and reliable rat model of in situ reversible obstructive jaundice with low morbidity and mortality rates. Rats were divided into 4 groups with 8 rats each: the sham-operated (SH) group only underwent laparotomy, the control internal drainage (ID-C) group underwent choledochoduodenostomy, the new internal drainage (ID-N) group and the long-term internal drainage (ID-L) group underwent choledochocholedochostomy. Common bile duct ligation was performed in all the drainage groups 7 days before reversal procedures. All rats were sacrificed for samples 7 days after the last operation except rats of the ID-L group that survived 28 days before sacrifice. Body weight, liver function, histopathological changes, morbidity and mortality were assessed. One rat died and 2 rats had complications with tube blockage in the ID-C group. No death or complications occurred in the ID-N and ID-L groups. The drainage tube remained patent in the long-term observation ID-L group. Body weight showed no significant difference between the ID-C and ID-N groups after 7 days drainage. Liver function was not fully recovered in the ID-C and ID-N groups after 7 days drainage, but statistical differences were only observed in the ID-C group compared with the SH and ID-L groups. Periportal inflammation and bile duct proliferation showed severer in the ID-C group than in the ID-N group. The present study provided an efficient, simple, and reliable rat model that is especially suitable for long-term or consecutive studies of reversible obstructive jaundice.