Reaction kinetics in intracellular environments with macromolecular crowding: simulations and rate laws
Reaction kinetics in intracellular environments with macromolecular crowding: simulations and rate laws
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DOI：
10.1016/j.pbiomolbio.2004.01.012
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发表时间：
2004-06-01
影响因子：
3.8
通讯作者：
Turner, TE
中科院分区：
文献类型：
--
作者：
Schnell, S;Turner, TE
我们回顾最近的证据，说明细胞质和试管生化动力学和热力学之间的根本区别，并显示在体内条件下的质量作用定律和幂律近似的故障。对非均匀介质中生化反应的模拟表明，由于生化物质的异常扩散和混合，反应遵循分形动力学。因此，传统的生化途径方程不能描述在体内条件下的反应。我们提出了一个修改后的Zipf-Mandelbrot分布，这将使拥挤的细胞内环境中发生的生化反应的建模和分析分形动力学。(C)2004爱思唯尔有限公司保留所有权利。
We review recent evidence illustrating the fundamental difference between cytoplasmic and test tube biochemical kinetics and thermodynamics, and showing the breakdown of the law of mass action and power-law approximation in in vivo conditions. Simulations of biochemical reactions in non-homogeneous media show that as a result of anomalous diffusion and mixing of the biochemical species, reactions follow a fractal-like kinetics. Consequently, the conventional equations for biochemical pathways fail to describe the reactions in in vivo conditions. We present a modification to fractal-like kinetics following the Zipf-Mandelbrot distribution which will enable the modelling and analysis of biochemical reactions occurring in crowded intracellular environments. (C) 2004 Elsevier Ltd. All rights reserved.