RuvAB and RecG are not essential for the recovery of DNA synthesis following UV-induced DNA damage in Escherichia coli
RuvAB and RecG are not essential for the recovery of DNA synthesis following UV-induced DNA damage in Escherichia coli
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DOI：
10.1534/genetics.166.4.1631
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发表时间：
2004-04-01
期刊：
影响因子：
3.3
通讯作者：
Courcelle, J
中科院分区：
文献类型：
--
作者：
Donaldson, JR;Courcelle, CT;Courcelle, J
紫外线诱导DNA损伤，阻断复制机制的进展。几个模型推测。在被UV诱导的DNA损伤破坏后复制的恢复需要新生DNA的消退或复制机制远离阻断损伤的迁移，以允许损伤的修复或绕过发生。RuvAB和RecG都在体外催化三链和四链DNA连接的分支迁移，并被提议在体内催化叉回归。为了研究这种可能性，我们表征了ruvAB和recG突变体中DNA合成的恢复。我们发现，在没有RecG或RuvAB的情况下，维持了被抑制的复制叉，并且DNA合成以与野生型细胞中的动力学相似的动力学恢复。本文提供的数据表明，RecG或RuvAB催化的叉回归对于DNA合成在体内UV诱导的DNA损伤后恢复不是必需的。
Ultraviolet light induces DNA lesions that block the progression of the replication machinery. Several models speculate that. the resumption of replication following disruption by UV-induced DNA damage requires regression of the nascent DNA or migration of the replication machinery away front the blocking lesion to allow repair or bypass of the lesion to occur. Both RuvAB and RecG catalyze branch migration of three- and four-stranded DNA junctions in vitro and are proposed to catalyze fork regression in vivo. To examine this possibility, we characterized the recovery of DNA synthesis in ruvAB and recG mutants. We found that in the absence of either RecG or RuvAB, arrested replication Forks are maintained and DNA synthesis is resumed with kinetics that are similar to those in wild-type cells. The data presented here indicate that RecG or RuvAB-catalyzed fork regression is not essential for DNA Synthesis to resume following arrest by UV-induced DNA damage in vivo.