Substrate Independent Liquid Phase Epitaxy of HKUST-1 as Anticoagulant and Antimicrobial Coating
Substrate Independent Liquid Phase Epitaxy of HKUST-1 as Anticoagulant and Antimicrobial Coating
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DOI：
10.1002/admi.201902011
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发表时间：
2020-04-30
影响因子：
5.4
通讯作者：
Weng, Yajun
中科院分区：
文献类型：
--
作者：
Zhang, Yu;Zhao, Qian;Weng, Yajun
在各类生物材料上有效沉积金属 - 有机框架仍然具有挑战性且非常必要。以聚多巴胺作为有效成核中心，在三种惰性生物材料上成功制备了HKUST - 1，这三种材料分别是氧化钛薄膜（Ti - O）、316L不锈钢（316L SS）和聚氯乙烯（PVC）。通过粉末X射线衍射对表面进行表征，证实了HKUST - 1在聚多巴胺涂层基底上的定向晶体生长。沉积有HKUST - 1的三个样品在催化一氧化氮生成和表面铜含量方面没有显著差异。它们都保持着约2.5×10⁻¹⁰ mol·cm⁻²·min⁻¹的一氧化氮释放速度，因此与未改性表面相比，HKUST - 1改性表面上的血小板活化受到抑制。体外实验表明血栓形成显著减少。HKUST - 1涂层表面使金黄色葡萄球菌和大肠杆菌的细菌附着减少了95%。内皮细胞和巨噬细胞培养结果表明改性表面具有良好的生物相容性。这为血液接触生物材料的表面改性提供了一种有前景、简便且有效的方法。
The effective deposition of metal-organic frameworks on kinds of biomaterials is still challenging and highly desirable. Using polydopamine as the effective nucleation center, HKUST-1 is successfully prepared on three kinds of inert biomaterials, i.e., titanium oxide film (Ti-O), 316L stainless steel (316L SS), and poly(vinyl chloride) (PVC). Characterization of the surfaces by powder X-ray diffraction confirm the oriented crystal growth of HKUST-1 on the polydopamine coated substrates. There are no significant difference in catalytical NO generation and surface copper content of the three samples with HKUST-1 deposition. All keep a NO release speed of approximate to 2.5 x 10(-10) mol cm(-2) min(-1), thus platelet activation on the HKUST-1 modified surfaces are inhibited compared to that of the unmodified ones. Ex vivo test shows thrombi formation is dramatically reduced. The HKUST-1 coated surfaces elicit 95% reduction in the bacterial attachment of both Staphylococcus aureus and Escherichia coli. The endothelial cells and macrophages culture results support good biocompatibility of the modified surfaces. It provides a promising, facile, and effective approach for surface modification of blood contact biomaterials.