Antibody-Based Detection of ERG Rearrangement-Positive Prostate Cancer
Antibody-Based Detection of ERG Rearrangement-Positive Prostate Cancer
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DOI：
10.1593/neo.10726
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发表时间：
2010-07-01
期刊：
影响因子：
4.8
通讯作者：
Rubin, Mark A.
中科院分区：
文献类型：
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作者：
Park, Kyung;Tomlins, Scott A.;Rubin, Mark A.
TMPRSS2-ERG基因融合发生在50%的前列腺癌中，并导致编码截短ERG产物的嵌合融合转录本的过度表达。以前检测截短的ERG产物的尝试因缺乏特异性抗体而受阻。在这里，我们通过免疫印迹分析、人工合成的TMPRSS2-ERG构建体、染色质免疫沉淀和免疫荧光，鉴定了兔抗ERG单抗(克隆EPR 3864；表观组学，伯林格姆，CA)。我们使用免疫组织化学(IHC)和荧光原位杂交(FISH)相结合的分析方法，将前列腺癌组织中ERG蛋白的表达与ERG基因重排的存在联系起来。我们独立评估了两个患者队列并观察到
TMPRSS2-ERG gene fusions occur in 50% of prostate cancers and result in the overexpression of a chimeric fusion transcript that encodes a truncated ERG product. Previous attempts to detect truncated ERG products have been hindered by a lack of specific antibodies. Here, we characterize a rabbit anti-ERG monoclonal antibody (clone EPR 3864; Epitomics, Burlingame, CA) using immunoblot analysis on prostate cancer cell lines, synthetic TMPRSS2-ERG constructs, chromatin immunoprecipitation, and immunofluorescence. We correlated ERG protein expression with the presence of ERG gene rearrangements in prostate cancer tissues using a combined immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) analysis. We independently evaluated two patient cohorts and observed