A perfect marker for fragrance genotyping in rice
A perfect marker for fragrance genotyping in rice
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DOI：
10.1007/s11032-005-0776-y
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发表时间：
2005-11-01
影响因子：
3.1
通讯作者：
Waters, DLE
中科院分区：
文献类型：
--
作者：
Bradbury, LMT;Henry, RJ;Waters, DLE
等位基因特异性扩增（阿萨）是一种低成本、稳健的技术，其可用于在单个PCR管内区分因SNP、插入或缺失而不同的等位基因。水稻的香味是一种隐性性状，已被证明是由于8号染色体上编码推定的甜菜碱醛脱氢酶2（BAD 2）的基因中的8个bp缺失和3个SNP所致。在这里，我们报告了一个单管阿萨测定，它允许芳香和非芳香水稻品种之间的歧视，并确定纯合芳香，纯合非芳香和杂合非芳香的个人在一个群体中分离的香味。外部引物产生约580 bp的片段作为每个样品的阳性对照。内部和相应的外部引物产生来自非芳香等位基因的355 bp片段和来自芳香等位基因的257 bp片段，允许在琼脂糖凝胶上进行简单分析。
Allele specific amplification (ASA) is a low-cost, robust technique that can be utilised to discriminate between alleles that differ by SNP's, insertions or deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has been shown to be due to an eight bp deletion and three SNP's in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2). Here we report a single tube ASA assay which allows discrimination between fragrant and non-fragrant rice varieties and identifies homozygous fragrant, homozygous non-fragrant and heterozygous non-fragrant individuals in a population segregating for fragrance. External primers generate a fragment of approximately 580 bp as a positive control for each sample. Internal and corresponding external primers produce a 355 bp fragment from a non-fragrant allele and a 257 bp fragment from a fragrant allele, allowing simple analysis on agarose gels.