Simple functionalization of cellulose beads with pre-propargylated chitosan for clickable scaffold substrates
Simple functionalization of cellulose beads with pre-propargylated chitosan for clickable scaffold substrates
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用于可点击支架基底的具有预炔丙基化壳聚糖的纤维素珠的简单功能化
DOI：
10.1007/s10570-021-03905-8
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发表时间：
2021-05-22
期刊：
影响因子：
5.7
通讯作者：
Peresin, Maria S.
中科院分区：
文献类型：
--
作者：
Gomez-Maldonado, Diego;Filpponen, Ilari;Peresin, Maria S.
鉴于生物基材料市场的增长以及环境友好型实践的需求，纤维素基微珠是更具吸引力的替代品之一。因此，本研究着重于采用一种相对简单直接的方法制备功能性纤维素基微珠，即在微珠形成之前，将带有目标官能团的预改性壳聚糖与纤维素混合，以此在形成的结构中引入官能团。为此，壳聚糖在均相反应条件下用溴丙炔进行化学改性，然后与氢氧化钠/尿素溶液中的纤维素混合，并在硝酸中凝固以制备球形微珠。通过元素分析以及傅里叶变换红外光谱、拉曼光谱和X射线光电子能谱对壳聚糖的成功化学改性进行了评估。壳聚糖衍生物中的炔基部分作为点击化学的反应性官能团，如通过在水介质中经由碘化亚铜催化的炔 - 叠氮环加成反应对商业荧光团叠氮 - 荧光488进行标记所证明的那样。这项工作展示了多种多糖用于功能性球形微珠的一步加工，作为生物基支架（如用于刺激响应应用和生物偶联的酶固定化）的模板。
Concerning the increased market for bio-based materials and environmentally safe practices, cellulose-based beads are one of the more attractive alternatives. Thus, this work focuses on the generation of functional cellulose-based beads with a relatively simple and direct method of blending a pre-modified chitosan bearing the targeted functional groups and cellulose, prior to the formation of the beads, as a mean to have functional groups in the formed structure. To this end, chitosan was chemically modified with propargyl bromide in homogenous reaction conditions and then combined with cellulose in sodium hydroxide/urea solution and coagulated in nitric acid to produce spherical shaped beads. The successful chemical modification of chitosan was assessed by elemental analysis, as well as by Fourier-transform infrared spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy. The alkynyl moieties from the chitosan derivative, served as reactive functional groups for click-chemistry as demonstrated by the tagging of the commercial fluorophore Azide-Fluor 488 via CuI-catalysed alkyne-azide cycloaddition reaction, in aqueous media. This work demonstrates the one-step processing of multiple polysaccharides for functional spherical beads as a template for bio-based scaffolds such as enzyme immobilization for stimuli-response applications and bioconjugations.