The distribution of RNA polymerase in Escherichia coli is dynamic and sensitive to environmental cues
The distribution of RNA polymerase in Escherichia coli is dynamic and sensitive to environmental cues
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DOI：
10.1046/j.1365-2958.2003.03805.x
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发表时间：
2003-12-01
影响因子：
3.6
通讯作者：
Jin, DJ
中科院分区：
文献类型：
--
作者：
Cabrera, JE;Jin, DJ
尽管对大肠杆菌RNA聚合酶（RNAP）进行了广泛的遗传、生化和结构研究，但对其在环境变化中的位置和分布知之甚少。用荧光显微镜观察E.在不同的生理条件下，构建了rpoC-gfp融合基因。我们发现，虽然RNAP位于类核和其周边，RNAP的分布是动态的，并受到细胞生长条件，营养饥饿和细胞内的整体转录活性的显着影响。此外，突变分析表明，稳定的RNA合成在类核凝聚中起着重要作用。
Despite extensive genetic, biochemical and structural studies on Escherichia coli RNA polymerase (RNAP), little is known about its location and distribution in response to environmental changes. To visualize the RNAP by fluorescence microscopy in E. coli under different physiological conditions, we constructed a functional rpoC-gfp gene fusion on the chromosome. We show that, although RNAP is located in the nucleoid and at its periphery, the distribution of RNAP is dynamic and dramatically influenced by cell growth conditions, nutrient starvation and overall transcription activity inside the cell. Moreover, mutational analysis suggests that the stable RNA synthesis plays an important role in nucleoid condensation.