Reactive oxygen species modulate the differentiation of neurons in clonal cortical cultures
Reactive oxygen species modulate the differentiation of neurons in clonal cortical cultures
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DOI：
10.1016/j.mcn.2006.08.005
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发表时间：
2006-12-01
影响因子：
3.5
通讯作者：
Crossin, Kathryn L.
中科院分区：
文献类型：
--
作者：
Tsatmali, Marina;Walcott, Elisabeth C.;Crossin, Kathryn L.
活性氧是细胞内信号传导的重要调节因子。我们研究了ROS在大鼠脑发育过程中的表达，并探讨了它们在皮质培养分化中的作用。在新生神经元中发现高水平的ROS。神经元产生ROS，不与细胞死亡，在整个胚胎发育和出生后阶段。到P20，仅在神经源性区域发现产生ROS的细胞。通过FRCS从E15脑中分离的具有低水平ROS的细胞在克隆培养物中分化为神经元、少突胶质细胞和星形胶质细胞。神经元在培养早期产生高活性氧，后来分化成两种类型：大的神经元样神经元，没有或只有一个单一的动作电位和较小的神经元，表达核钙视网膜蛋白和发射重复的动作电位。抗氧化剂处理没有改变神经元的数量，但增加了小到大的神经元的比例。这些发现表明ROS水平的调节影响神经元分化的多个方面。(c)2006年爱思唯尔公司All rights reserved.
Reactive oxygen species (ROS) are important regulators of intracellular signaling. We examined the expression of ROS during rat brain development and explored their role in differentiation using cortical cultures. High levels of ROS were found in newborn neurons. Neurons produced ROS, not connected with cell death, throughout embryogenesis and postnatal stages. By P20, ROS-producing cells were found only in neurogenic regions. Cells with low levels of ROS, isolated from E15 brains by FRCS, differentiated into neurons, oligodendrocytes, and astrocytes in clonal cultures. Neurons produced high ROS early in culture and later differentiated into two types: large pyramidal-like neurons that fired no or only a single action potential and smaller neurons that expressed nuclear calretinin and fired repeated action potentials. Antioxidant treatment did not alter neuron number but increased the ratio of small to large neurons. These findings suggest that modulation of ROS levels influences multiple aspects of neuronal differentiation. (c) 2006 Elsevier Inc. All rights reserved.