Correlation between miR-148 Expression in Vitreous and Severity of Rhegmatogenous Retinal Detachment.
Correlation between miR-148 Expression in Vitreous and Severity of Rhegmatogenous Retinal Detachment.
复制标题
DOI：
10.1155/2017/3427319
复制
发表时间：
2017
影响因子：
--
通讯作者：
Terasaki H
中科院分区：
文献类型：
--
作者：
Tsunekawa T;Kaneko H;Takayama K;Hwang SJ;Oishi A;Nagasaka Y;Ye F;Iwase T;Nonobe N;Ueno S;Ito Y;Yasuda S;Matsuura T;Shimizu H;Suzumura A;Kataoka K;Terasaki H
目的.我们之前曾报道过hsa-miR-148 a-3 p在孔源性视网膜脱离（RRD）眼中呈阳性表达，并参与体外视网膜色素上皮的上皮-间充质转化。我们研究了RRD患者玻璃体液中hsa-miR-148 a-3 p表达水平与RRD严重程度的关系。方法.在27只患有RRD的眼睛中测量玻璃体液中hsa-miR-148 a-3 p的表达水平、视网膜脱离（RD）的范围（程度）和视网膜裂孔的像素。多元回归分析hsa-miR-148 a-3 p表达水平与其他因素的相关性。结果hsa-miR-148 a-3 p表达水平、从RRD发作到玻璃体切除术的时间、RD范围和视网膜裂孔像素分别为23.68 ± 43.00、12.07 ± 15.36天、155.85 ± 86.67度和37000 ± 67100像素。5例RRD术前有玻璃体积血。hsa-miR-148 a-3 p表达水平与视网膜裂孔像素（β = 0.699）和从RRD发作到玻璃体切除术的时间（β = 0.358）显著相关，但与RD的范围或玻璃体出血的存在无关。结论玻璃体液中hsa-miR-148 a-3 p的表达水平与视网膜裂孔的大小和病程显著相关。
Purpose. We had earlier reported positive hsa-miR-148a-3p expression in eyes with rhegmatogenous retinal detachment (RRD) and its involvement in the epithelial-mesenchymal transition of retinal pigment epithelium in vitro. Here we investigated the association of hsa-miR-148a-3p expression levels in the vitreous fluid of patients with RRD with severity of RRD. Methods. The hsa-miR-148a-3p expression levels in the vitreous fluid, range (degree) of retinal detachment (RD), and pixels of retinal break were measured in 27 eyes with RRD. The association of hsa-miR-148a-3p expression levels with other factors was evaluated by multiple regression analysis. Results. The hsa-miR-148a-3p expression levels, time from onset of RRD to vitrectomy, range of RD, and pixels of retinal breaks were 23.68 ± 43.00, 12.07 ± 15.36 days, 155.85 ± 86.67 degrees, and 37000 ± 67100 pixels, respectively. Five eyes with RRD had vitreous hemorrhage preoperatively. The hsa-miR-148a-3p expression levels were significantly associated with pixels of retinal breaks (β = 0.699) and the time from onset of RRD to vitrectomy (β = 0.358) but not with the range of RD or presence of vitreous hemorrhage. Conclusion. The hsa-miR-148a-3p expression levels in the vitreous fluid were significantly associated with the size of retinal break and disease duration.