AMINO-ACID SUBSTITUTIONS IN MITOCHONDRIAL ATPASE SUBUNIT-9 OF SACCHAROMYCES-CEREVISIAE LEADING TO OLIGOMYCIN OR VENTURICIDIN RESISTANCE
AMINO-ACID SUBSTITUTIONS IN MITOCHONDRIAL ATPASE SUBUNIT-9 OF SACCHAROMYCES-CEREVISIAE LEADING TO OLIGOMYCIN OR VENTURICIDIN RESISTANCE
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DOI：
10.1016/0014-5793(86)80152-1
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发表时间：
1986-01-20
期刊：
影响因子：
3.5
通讯作者：
OOI, PG
中科院分区：
文献类型：
--
作者：
NAGLEY, P;HALL, RM;OOI, PG
本文报道了一系列对异源寡霉素具有抗性的酵母菌突变株的研究结果。该基因的DNA序列分析已被用于确定线粒体ATP酶复合物亚基9中的氨基酸取代。可以识别参与寡霉素抗性的氨基酸结构域，其包括亚基9多肽的两个疏水部分中的每一个中的残基，所述疏水部分被认为跨越线粒体内膜。某些氨基酸取代也赋予对venturicidin的交叉抗性：这些残基定义了venturicidin抗性的内部结构域。由一个特定取代产生的venturiicidin抗性的表达受核遗传因子调节。
A series of isonuclear oligomycin-resistant mutants ofSaccharomyces cerevisiaecarrying mutations in the mitochondrialolilgene has been studied. DNA sequence analysis of this gene has been used to define the amino acid substitutions in subunit 9 of the mitochondrial ATPase complex. A domain of amino acids involved in oligomycin resistance can be recognized which encompasses residues in each of the two hydrophobic portions of the subunit 9 polypeptide that are thought to span the inner mitochondrial membrane. Certain amino acid substitutions also confer cross-resistance to venturicidin: these residues define an inner domain for venturicidin resistance. The expression of venturicidin resistance resulting from one particular substitution is modulated by nuclear genetic factors.