Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys
Optimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys
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DOI：
10.1002/jbm.b.33668
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发表时间：
2017-08-01
影响因子：
3.4
通讯作者：
Stevens, M. M.
中科院分区：
文献类型：
--
作者：
He, M.;Callanan, A.;Stevens, M. M.
肾移植已被公认为肾脏替代疗法的最佳形式，但受到可用于移植的器官库有限的限制。整个器官去细胞化方法正在引领再生医学解决方案走向生物工程器官替代。然而，在任何潜在的临床应用之前，去细胞化和再细胞化参数的系统预优化是必不可少的，并且应该是整个器官去细胞化作为生物工程器官替代物的潜在策略的下一个阶段。在这里，我们系统地评估了两个基本参数（浓度和灌注持续时间）的影响，不同的暴露于最常用的单一脱细胞剂（十二烷基硫酸钠/SDS）在灌注脱细胞过程中的整个大鼠肾脏ECM生物支架，结果显示，改善保存的结构和功能成分的整个肾脏ECM生物支架。基于我们的增强方案的全肾生物支架成功地与大鼠原代肾细胞和间充质基质细胞再细胞化。这些研究结果应广泛适用于脱细胞的整个器官生物支架及其优化的再生器官移植替代品的发展。(C)2016 Wiley Periodicals，Inc.
Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization parameters is essential prior to any potential clinical application and should be the next stage in the evolution of whole organ decellularization as a potential strategy for bioengineered organ replacements. Here we have systematically assessed two fundamental parameters (concentration and duration of perfusion) with regards to the effects of differing exposure to the most commonly used single decellularizing agent (sodium dodecyl sul-phate/SDS) in the perfusion decellularization process for whole rat kidney ECM bioscaffolds, with findings showing improved preservation of both structural and functional components of the whole kidney ECM bioscaffold. Whole kidney bioscaffolds based on our enhanced protocol were successfully recellularized with rat primary renal cells and mesenchymal stromal cells. These findings should be widely applicable to decellularized whole organ bioscaffolds and their optimization in the development of regenerated organ replacements for transplantation. (C) 2016 Wiley Periodicals, Inc.