Time domain diffuse Raman spectrometer based on a TCSPC camera for the depth analysis of diffusive media.
Time domain diffuse Raman spectrometer based on a TCSPC camera for the depth analysis of diffusive media.
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DOI：
10.1364/ol.43.002134
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发表时间：
2018-05
期刊：
影响因子：
3.6
通讯作者：
S. Sekar;S. Mosca;S. Tannert;G. Valentini;F. Martelli;T. Binzoni;Y. Prokazov;E. Turbin;W. Zuschratter;R. Erdmann;A. Pifferi
中科院分区：
文献类型：
--
作者：
S. Sekar;S. Mosca;S. Tannert;G. Valentini;F. Martelli;T. Binzoni;Y. Prokazov;E. Turbin;W. Zuschratter;R. Erdmann;A. Pifferi
提出了一种用于高散射介质深度探测的时域漫拉曼光谱仪。该系统是基于，据我们所知，一种新的时间相关的单光子计数（TCSPC）相机，同时获得光谱和时间信息的拉曼光子。建立了专用的非接触式探头，并在组织模拟双层体模上进行时域拉曼测量。通过早期时间门控（0-212 ps）拉曼光子来消除拉曼信号的荧光污染。深度灵敏度是通过时间门控拉曼光子在不同的延迟与106 ps的门宽度。重要的是，时域可以提供依赖于时间的深度灵敏度，导致两层拉曼信号之间的高对比度。结果，我们的双层体模的增强因子为2170，远高于空间偏移拉曼光谱（SORS）、频率偏移拉曼光谱（FORS）或混合FORS-SORS在类似体模上获得的值。
We present a time domain diffuse Raman spectrometer for depth probing of highly scattering media. The system is based on, to the best of our knowledge, a novel time-correlated single-photon counting (TCSPC) camera that simultaneously acquires both spectral and temporal information of Raman photons. A dedicated non-contact probe was built, and time domain Raman measurements were performed on a tissue mimicking bilayer phantom. The fluorescence contamination of the Raman signal was eliminated by early time gating (0-212 ps) the Raman photons. Depth sensitivity is achieved by time gating Raman photons at different delays with a gate width of 106 ps. Importantly, the time domain can provide time-dependent depth sensitivity leading to a high contrast between two layers of Raman signal. As a result, an enhancement factor of 2170 was found for our bilayer phantom which is much higher than the values obtained by spatial offset Raman spectroscopy (SORS), frequency offset Raman spectroscopy (FORS), or hybrid FORS-SORS on a similar phantom.