PATCH-CLAMP TECHNIQUES FOR TIME-RESOLVED CAPACITANCE MEASUREMENTS IN SINGLE CELLS
PATCH-CLAMP TECHNIQUES FOR TIME-RESOLVED CAPACITANCE MEASUREMENTS IN SINGLE CELLS
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DOI：
10.1007/bf00582306
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发表时间：
1988-02-01
影响因子：
4.5
通讯作者：
NEHER, E
中科院分区：
文献类型：
--
作者：
LINDAU, M;NEHER, E
本文介绍了两种在密闭全细胞记录中估算被动细胞参数的方法，如膜电容、膜电导和通路电阻。这两种方法都限制在其应用的情况下，所研究的细胞可以近似由一个简单的三分量网络与线性特性在一定的电压范围内。一种方法，称为时域技术，只需要标准的电生理设备和计算机。 参数来自方波刺激期间电容瞬变的分析。它很容易适应实验参数的广泛变化。特别地，其同样适用于“慢全细胞”配置（接入电阻在100 M Ω范围内）。至1 G Ω）以及正常的全细胞测量（接入电阻通常为10 M Ω）。另一种方法将正弦波命令信号施加到电池，并采用锁定放大器来分析所得的电流信号。描述了锁定放大器的两种操作模式。一种模式提供与最大分辨率（1-10 fF）下电容的微小变化成正比的输出信号。另一种模式，与数字计算机相结合，提供所有无源电池参数的估计，如时域技术，但大量的数据减少由锁定放大器本身执行。但由于硬件的特殊性，这种方法不如时域技术灵活。
Two methods are described for estimation of passive cell parameters such as membrane capacitance, membrane conductance and access resistance in tight-seal whole cell recording. Both methods are restricted in their application to cases where the cell under under study can be approximated by a simple three-component network with linear properties over some voltage range. One method, referred to as the time domain technique, requires only standard electrophysiological equipment and a computer. Parameters are derived from an analysis of capacitive transients during square wave stimulation. It is readily adaptable to wide variations in experimental parameters. Particularly, it is equally applicable to the "slow whole-cell" configuration (access resistance in the range 100 M.OMEGA. to 1 G.OMEGA.) and to normal whole-cell measurements (access resistance typically 10 M.OMEGA.). The other method applies a sine wave command signal to the cell and employs a lock-in amplifier to analyse the resulting current signal. Two modes of operating the lock-in amplifier are described. One mode provides an output signal directly proportional to small changes in capacitance at maximum resolution (1-10 fF). The other mode, in conjunction with a digital computer, supplies estimates of all passive cell parameters, as does the time domain technique, but with a large amount of data reduction performed by the lock-in amplifier itself. Due to the special hardware, however, this method is not as flexible as the time domain technique.