Comparison of marine sediment toxicity test protocols for the amphipod Rhepoxynius abronius and the polychaete worm Nereis (Neanthes) arenaceodentata
Comparison of marine sediment toxicity test protocols for the amphipod Rhepoxynius abronius and the polychaete worm Nereis (Neanthes) arenaceodentata
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DOI：
10.1002/etc.5620170513
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发表时间：
1998-05-01
影响因子：
4.1
通讯作者：
Tjeerdema, RS
中科院分区：
文献类型：
--
作者：
Anderson, BS;Hunt, JW;Tjeerdema, RS
比较了长脚类动物10 d生存毒性试验方案和多毛类动物20 d生长和生存毒性试验方案。在加州收集的341份沉积物样品中，78%的样品显著抑制了棘齿鼠的生存，而26%的样品显著抑制了沙齿鼠的生存和生物量。通过计算该数据集的每个测试协议的最小显著差异（MSD），确定与每个协议端点相关的统计能力。粗齿田鼠和砂齿田鼠的生存和生物量的90百分位MSDs分别为16%、36%和56%。金属、农药、多环芳烃、多氯联苯等多种有毒物质对黑腹夜蛾的存活率有显著的负相关影响。arenaceententata的存活率或生物量与测量的污染物之间没有显著的相关性。片脚类动物的存活率也与沉积物总有机碳和颗粒尺寸呈负相关，但当高颗粒尺寸（bbb90 %）的样品从考虑中剔除时，研究的总体结论没有改变。10 d的片足类动物生存试验是一个更敏感的毒性指标，但结果表明，这是由于更大的统计能力，而不是由于测试生物或终点的更大敏感性。
The 10-d amphipod survival toxicity test protocol using Rhepoxynius abronius was compared to the 20-d polychaete worm growth and survival protocol using Nereis (Neanthes) arenaceodentata. Of the 341 sediment samples collected in California and tested over a 2-year period, 78% significantly inhibited R. abronius survival, whereas 2 and 26% significantly inhibited N. arenaceodentata survival and biomass, respectively. Statistical power associated with each protocol endpoint was determined,by calculating the minimum significant difference (MSD) for each test protocol for this data set. The 90th percentile MSDs for R. abronius survival and N. arenaceodentata survival and biomass were 16, 36, and 56%, respectively. Survival of R. abronius was significantly negatively correlated with a number of toxicants including metals, pesticides, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls. No significant correlations were determined between N. arenaceodentata survival or biomass and contaminants measured. Amphipod survival was also negatively correlated with sediment total organic carbon and grain size, but when samples with a high percent fine grain size (>90% fines) were eliminated from consideration, the overall conclusions of the study were not altered. The 10-d amphipod survival protocol using R. abronius was a more sensitive indicator of toxicity, but the results indicate that this was due to greater statistical power rather than greater sensitivity of the test organisms or endpoints.