[Qualitative and quantitative determination of the main components of huanglianjiedu decoction by HPLC-UV/MS].
[Qualitative and quantitative determination of the main components of huanglianjiedu decoction by HPLC-UV/MS].
复制标题
DOI：
--
复制
发表时间：
2006-04
期刊：
影响因子：
--
通讯作者：
Jian Sun;Ji-sheng Ma;Jin Jin-Jin;Huai-sheng Wang;Qing-hui Wen;Hong-gui Zhang;Qiu-li Zhou
中科院分区：
文献类型：
--
作者：
Jian Sun;Ji-sheng Ma;Jin Jin-Jin;Huai-sheng Wang;Qing-hui Wen;Hong-gui Zhang;Qiu-li Zhou
目的建立黄连解毒汤的高效液相色谱分析方法。方法采用HPLC-UV/MS联用技术对黄连解毒汤全草及单味药材的化学成分进行鉴定。Zorbax Extend C18（150 mm x 4. 6 mm ID，5 microm）柱;移动的相由乙腈（A）和水（B，含0.5%乙酸）组成，梯度洗脱;流速为1.0 mL × min（-1），柱温设定为25 ℃。检测波长为254 nm。结果黄连解毒汤的色谱图中共有21个主要峰。其中，峰1、2、5、18为栀子的峰，峰8、13、14、15、16、17、19、21为黄芩的峰。黄连10株，黄檀20株，峰3、4、6、9、11和12来自它们的组合。除栀子外，其余药材均出现峰7。通过保留时间、在线紫外光谱和质谱的比较，确定了11个峰，（geniposide），9（jatrorrhizine），10其中黄连碱11（巴马汀）12（小檗碱）13（黄芩苷）15（槲皮素A）17（汉黄芩苷）19（黄芩素）20（obaculactone）21（汉黄芩素）15（黄连碱）11（巴马汀）12（小檗碱）13（黄芩苷）15（槲皮素A）17（汉黄芩苷）19（黄芩素）20（obaculactone）21（汉黄芩素）21（汉黄芩素）。结论该方法能较好地反映黄连解毒汤的特征，可用于黄连解毒汤的质量控制。对黄连和黄檀进行了鉴别。HPLC首次
AIM To establish a comprehensive HPLC analytical method of Huanglianjiedu decoction. METHODS This study was performed by HPLC-UV/MS to identify the chemical constituents of the whole and individual herbs of the "Huanglianjiedu decoction". Zorbax Extend C18 (150 mm x 4. 6 mm ID, 5 microm) column was used; the mobile phase was composed of acetonitrile (A) and water (B, with 0.5% acetic acid) with gradient elution; the flow rate was 1.0 mL x min(-1) and the column temperature was setup at 25 degrees C. The detection wavelength was 254 nm. RESULTS The chromatogram of Huanglianjiedu decoction showed 21 main peaks. Peaks 1, 2, 5 and 18 were from Gardenia jasminoides Ellis, Peaks 8, 13, 14, 15, 16, 17, 19 and 21 from Scutellaria baicalensis Georgi. While 10 from Coptis chinensis Franch and 20 from Phellodendron amurense Rupr., Peaks 3, 4, 6, 9, 11 and 12 came from them together. Peak 7 presented in the chromatograms of the herbs except Gardenia jasminoides Ellis. By comparison of the retention time, the on-line UV spectra and MS spectra, 11 peaks were identified as 5 (geniposide), 9 (jatrorrhizine), 10 (coptisine), 11 (palmatine), 12 (berberine), 13 (baicalin), 15 (oroxin A), 17 (wogonoside), 19 (baicalein), 20 (obaculactone), 21 (wogonin), then eight of them were quantified by HPLC-UV. CONCLUSION The method could represent the characteristics of Huanglianjiedu decoction, and it could be used to evaluate the quality and quantity of Huanglianjiedu decoction. It distinguished between Coptis chinensis Franch and Phellodendron amurense Rupr. by HPLC for the first time.