Insulin-like growth factor-I improves cellular and molecular aspects of healing in a collagenase-induced model of flexor tendinitis
Insulin-like growth factor-I improves cellular and molecular aspects of healing in a collagenase-induced model of flexor tendinitis
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DOI：
10.1016/s0736-0266(02)00009-8
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发表时间：
2002-09-01
影响因子：
2.8
通讯作者：
Nixon, AJ
中科院分区：
文献类型：
--
作者：
Dahlgren, LA;van der Meulen, MCH;Nixon, AJ
屈肌腱炎是一种常见的和衰弱的伤害精英和休闲运动员。通过肌腱内注射胰岛素样生长因子-I（IGF-I）可以改善愈合，体外研究表明IGF-I可以刺激有丝分裂和增强肌腱基质的产生。本研究调查了在所有屈肌腱炎的马模型中腱内注射IGF-I油肌腱愈合的效果。胶原酶诱导的病变中创建的两个前肢的趾浅屈肌腱的拉伸区域的8匹马。治疗组肌腱每隔一天病灶内注射2 μ g rhIGF-I，共注射10次，对照组注射0.9%NaCl。在8周的试验期间，使用超声连续评价肌腱纤维沉积和组织。安乐死后，收获肌腱和DNA，羟脯氨酸，和糖胺聚糖含量测定，机械强度和刚度评价，基因表达和空间排列的胶原蛋白I型和III型评估北方印迹和原位杂交，和肌腱纤维结构偏振光显微镜评估。IGF-I治疗肢体的局部软组织肿胀减轻。同样，IGF-1治疗肌腱的病变大小在治疗开始后3周和4周较小。与对照组相比，IGF-I处理的肌腱的细胞增殖和胶原蛋白含量增加。在机械上，IGF-I处理的肌腱与盐水处理的对照相比显示出刚度增加的趋势。考虑到软组织肿胀的减少和超声愈合的改善，这些数据支持病灶内IGF-1治疗衰弱性肌腱损伤的潜在用途。(C)2002骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
Flexor tendinitis is a common and debilitating injury of elite and recreational athletes. Healing may be improved through intratendinous injection of insulin-like growth factor-I (IGF-I), which has been shown in vitro to stimulate mitogenesis and enhance tendon matrix production. This Study investigated the effects of intratendinous injection of IGF-I oil tendon healing in all equine model of flexor tendinitis. Collagenase-induced lesions were created in the tensile region of the flexor digitorum superficialis tendon of both forelimbs of eight horses. Treated tendons were injected with 2 mug rhIGF-I intralesionally every other day for 10 injections, while controls received 0.9% NaCl. Tendon fiber deposition and organization were evaluated serially using ultrasonography throughout the 8 week trial period. Following euthanasia, the tendons were harvested and DNA, hydroxyproline, and glycosaminoglycan content determined, mechanical strength and stiffness evaluated, gene expression and spatial arrangement of collagen types I and III assessed by northern blot and in situ hybridization, and tendon fiber architecture assessed by polarized light microscopy. Local soft tissue swelling was reduced in the IGF-I treated limbs. Similarly, lesion size in IGF-1 treated tendons was smaller 3 and 4 weeks after initiation of treatment. Cell proliferation and collagen content of the IGF-I treated tendons were increased compared to controls. Mechanically, IGF-I treated tendons showed a trend toward increased stiffness compared to saline treated controls. Considered together with the decreased soft tissue swelling and improved sonographic healing, these data support the potential use of intralesional IGF-1 for treatment of debilitating tendon injuries. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.