Effect of the polymorphism of prolactin receptor (PRLR) and leptin (LEP) genes on litter size in Polish pigs.
Effect of the polymorphism of prolactin receptor (PRLR) and leptin (LEP) genes on litter size in Polish pigs.
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DOI：
10.1111/j.1439-0388.2005.00547.x
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发表时间：
2005-12
期刊：
影响因子：
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通讯作者：
A. Terman
中科院分区：
文献类型：
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作者：
A. Terman
本实验的目的是使用PRLR和LEP基因中的DNA突变来确定波兰大白色×长白猪中基因型和产仔数之间的关联。研究的繁殖性状包括：总产仔数（TNB）、活产仔猪数（NBA）和断奶仔猪数。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，以PRLR和LEP基因为研究对象，采用特异性引物和限制性内切酶分别检测PRLR和LEP基因的多态性。PRLR和LEP基因的等位基因分别为A（0.62）和B（0.38），LEP基因的等位基因分别为C（0.10）和T（0.90）。分析PRLR和LEP基因型与总氮、NBA和NW的关系。分析显示，在第一次产次母猪中，携带不同PRLR基因型的母猪之间存在统计学显著（p <或= 0.01）差异。在以后的产次中，AA基因型母猪与AB和BB型母猪相比仍具有最大的产仔数，但差异不显著。对产率x PRLR相互作用的分析显示差异较小且无统计学显著性。不同LEP基因型与TNB、NBA、NW的相关性分析显示，差异较小且无统计学意义。
The aim of the experiment was to use the DNA mutations in the PRLR and LEP genes to determine associations between the genotype and litter size in Polish Large White x Landrace sows. Reproductive traits investigated were: total number of piglets born (TNB), number of piglets born alive (NBA) and number of piglets weaned. The polymorphism in PRLR and LEP genes was detected using the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method, with specific primers and the restriction enzymes AluI and HinfI respectively. Two different alleles of PRLR and LEP gene were identified: alleles A (0.62) and B (0.38) of the PRLR gene and alleles C (0.10) and T (0.90) of the LEP gene. The relationships between the PRLR and LEP genotypes and TBN, NBA and NW were analysed. The analysis showed, in first parity sows, statistically significant (p < or = 0.01) differences between sows carrying different PRLR genotypes. In later parities, sows with the AA genotype still had the largest litter size compared with AB and BB sows, but the difference was statistically not significant. Analysis of the interaction PARITY x PRLR showed small and statistically not significant differences. The analysis of relationship between different LEP genotypes and TNB, NBA, NW showed small and statistically non-significant differences.