Effect of Triton X-100 on the removal of aqueous phenol by laccase analyzed with a combined approach of experiments and molecular docking
Effect of Triton X-100 on the removal of aqueous phenol by laccase analyzed with a combined approach of experiments and molecular docking
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通过实验和分子对接相结合的方法分析 Triton X-100 对漆酶去除水中苯酚的影响
DOI：
10.1016/j.colsurfb.2012.04.001
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发表时间：
2012-09-01
影响因子：
5.8
通讯作者：
Xie, Gengxin
中科院分区：
文献类型：
--
作者：
Zhang, Yu;Zeng, Zhuotong;Xie, Gengxin
研究了Triton X-100对漆酶催化去除苯酚的影响。当苯酚和漆酶的浓度分别为50 mg/L和0.05 mg/mL时，Triton X-100的最佳浓度为155 μ M。漆酶活性增加与Triton X-100的浓度从31到930 μ M和最高的增加是约17%，由930 μ M Triton X-100。当苯酚初始浓度分别为50、100、200、400和600 mg/L时，155 μ M Triton X-100对苯酚的去除效率分别是对照的1.2、1.6、3.4、4.5和5.7倍。利用分子对接方法研究了漆酶与底物的相互作用。对接结果表明，苯酚与漆酶之间形成了氢键和疏水作用，而Triton X-100与漆酶之间形成了疏水作用，这可能会提高漆酶的活性，从而促进苯酚的去除。利用紫外光谱对苯酚去除反应进行了表征。结果表明，低浓度的Triton X-100的存在下，苯酚的酶催化去除可能是一种替代目前的苯酚去除工艺在水处理或修复。(C)2012爱思唯尔有限公司版权所有。
Effects of Triton X-100 on the removal of aqueous phenol catalyzed by laccase were studied. The optimal concentration of Triton X-100 was 155 mu M to improve phenol removal when the concentrations of phenol and laccase were 50 mg/L and 0.05 mg/mL, respectively. Laccase activity was increased with Triton X-100 at concentrations from 31 to 930 mu M and the highest increase was about 17% by 930 mu M Triton X-100. The removal efficiencies of phenol with 155 mu M Triton X-100 were 1.2, 1.6, 3.4, 4.5, and 5.7 fold those of the control after Oh when the initial concentrations of phenol were 50, 100, 200, 400 and 600 mg/L, respectively. Molecular docking method was used to analyze the interactions between laccase and substrates. Docking results showed that phenol formed hydrogen bonds and hydrophobic interactions with laccase, whereas Triton X-100 formed hydrophobic interactions with laccase, which may increase the laccase activity and enhance phenol removal. The reaction of phenol removal was also characterized using UV spectra. The results indicated that the presence of low concentrations of Triton X-100 for phenol removal catalyzed by enzymes may be an alternative to the present phenol removal processes in water treatment or remediation. (C) 2012 Elsevier B.V. All rights reserved.