Establishment of a bovine leukemia virus-free dairy herd in Korea.
Establishment of a bovine leukemia virus-free dairy herd in Korea.
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在韩国建立无牛白血病病毒的奶牛群。
DOI：
10.4142/jvs.2005.6.3.227
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发表时间：
2005
影响因子：
1.8
通讯作者：
Chung
中科院分区：
文献类型：
--
作者：
G. Suh;J. Lee;Chai;T. Hur;D. Son;B. Ahn;Nam Chul Kim;Chung
考虑到全国牛白血病病毒（BLV）的感染率很高，选择了忠南道的一个大型奶牛场，并实施了“检测和隔离”计划。1999年7月，对该农场491头牛进行了酶联免疫吸附试验，只有163头牛（139头成年牛、18头母牛和6头公牛）blv血清阴性。从2000年2月到2004年4月，将血清阴性组放置在距离血清阳性组1500至2000米的谷仓中，此后每隔3至5个月用ELISA进行检测。在连续试验中血清转化的动物在检测到blv抗体后立即从血清阴性组中取出。管理上的改变旨在防止牛之间的医源性血液转移。从其他国家进口的替代小牛和在农场出生的小牛通过ELISA反复检测，只有血清阴性的动物被引入该组。截至2004年4月，该农场有311头牛BLV血清阴性。最初的139头成年奶牛中有24头在2000年进行了血清转化，自2001年2月以来没有发现血清阳性的动物。对该组的随访显示，三年内没有BLV感染复发，所有血清阳性的牛都被转移到一个单独的地方。本研究中的方法可能对想要走向blv阴性状态的韩国生产商有价值。
In view of the high prevalence rate of bovine leukemia virus (BLV) infections in cattle over the entire country, a large dairy farm in Chungnam province was chosen and 'test and segregate' program was instituted. On July 1999, ELISA test was performed on 491 animals on the farm and only 163 cattle (139 adult cows, 18 female and 6 male calves)were BLV-seronegative. From February 2000 through April 2004, the seronegative group was placed in barns 1,500 to 2,000 m from seropositive group and thereafter tested at 3-to 5-month intervals by ELISA. Animals seroconverted in consecutive tests were removed from the seronegative group immediately after the detection of anti-BLV antibodies. The changes in management were aimed at preventing iatrogenic transfer of blood between cattle. Replacement heifers imported from other countries and calves born at the farm were repeatedly tested by ELISA, and only seronegative animals were introduced into the group. As of April 2004, there were 311 cattle in the BLV seronegative group of the farm. Twenty four cows of the initial 139 adult cows were seroconverted in 2000, and no seropositive animals were found since February 2001. Follow up of the group, from which all seropositive cattle were moved to a separate location, revealed no recurrence of BLV infection for three years. The approach in the present study might be valuable for Korean producers who would like to move toward a BLV-negative status.