Cutaneous expression of thymic stromal lymphopoietin (TSLP) in vitiligo patients: a case-control study
Cutaneous expression of thymic stromal lymphopoietin (TSLP) in vitiligo patients: a case-control study
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DOI：
10.1111/ijd.14282
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发表时间：
2019-05-01
影响因子：
3.6
通讯作者：
El Darbi, Reem
中科院分区：
文献类型：
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作者：
Elmasry, Maha Fathy;Nagui, Noha Abdel Rehim;El Darbi, Reem
胸腺基质淋巴生成素（TSLP）是一种促进T - helper-2反应的主要促过敏细胞因子。我们的目的是研究和验证TSLP在白癜风发病机制中的作用。方法本病例对照研究选取25例广泛性非节段性白癜风患者（招募自开罗大学医学院Kasr El Ainy皮肤科门诊）和25例符合纳入标准的健康对照者，为期7个月（2017年1月- 2017年7月）。患者接受完整的病史、白癜风的详细评估和照片记录。从患者的白癜风皮肤和对照组的正常皮肤背部进行皮肤活检，使用定量实时聚合酶链反应技术测量TSLP信使RNA （mRNA）组织的相对表达水平。结果患者TSLP mRNA表达量与对照组比较差异有统计学意义（P < 0.001），对照组TSLP mRNA表达量较对照组低。结论TSLP mRNA在白癜风皮肤中的表达水平低于正常皮肤，提示TSLP在白癜风发病过程中发挥着重要作用。
Background Thymic stromal lymphopoietin (TSLP) is a major pro-allergic cytokine promoting T helper-2 responses. Our aim was to study and verify the hypothesis of the role of TSLP in the pathogenesis of vitiligo. Methods This case-control study was conducted on 25 patients with generalized non-segmental vitiligo (recruited from the Dermatology outpatient clinic, Kasr El Ainy, Faculty of Medicine, Cairo University) and 25 healthy controls fulfilling the inclusion criteria over a period of 7 months (January 2017-July 2017). Patients underwent complete medical history, detailed assessment of vitiligo, and photographic documentation. Skin biopsies were taken from the back from both patients' vitiliginous skin and from normal skin of controls for which relative TSLP messenger RNA (mRNA) tissue expression levels were measured using quantitative real-time polymerase chain reaction technique. Results There was a statistically significant difference between the TSLP mRNA expression levels in patients and controls (P < 0.001) with lower levels in the former group. Conclusion This study revealed lower TSLP mRNA expression levels in vitiliginous skin than in normal skin suggesting an imminent role of TSLP in the pathogenesis of vitiligo.