Detection of coronavirus in vampire bats (Desmodus rotundus) in southern Brazil
Detection of coronavirus in vampire bats (Desmodus rotundus) in southern Brazil
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DOI：
10.1111/tbed.14150
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发表时间：
2021-05-28
影响因子：
4.3
通讯作者：
Canal, Claudio Wageck
中科院分区：
文献类型：
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作者：
Alves, Raquel Silva;do Canto Olegario, Juliana;Canal, Claudio Wageck
吸血蝙蝠（Desmodus rotundus）是一种吸血动物，专门以家养哺乳动物的血液为食。吸血蝙蝠的进食习惯使它们能够与哺乳动物宿主接触，并可能增加人畜共患病的溢出。此外，它们可能携带几种致病生物，包括冠状病毒（CoV），它们是重要的宿主。引起严重急性呼吸综合征（SARS-CoV），中东呼吸综合征（MERS-CoV）和可能的2019年冠状病毒病（SARS-CoV-2）的人类病原体都起源于蝙蝠，但需要桥梁宿主才能传播到人群中。为了监测蝙蝠中潜在的人畜共患病毒的存在，目前的工作评估了巴西南部吸血蝙蝠中CoV的存在。2017年至2019年期间，巴西南部南里奥格兰德州共有101只吸血蝙蝠被捕获并实施安乐死。收集脑、心脏、肝脏、肺、肾脏和肠，并分别浸软。将样品合并并使用Illumina MiSeq平台进行高通量测序（HTS），随后使用泛冠状病毒RT-PCR方案进行单独筛选。我们在HTS中检测到CoV相关序列，但通过RT-PCR仅2只（2/101; 1.98%）动物在肠道中检测到CoV。在同一样品中获得了RdRp和spike基因的部分序列，在另一样品中获得了RdRp区域。这些序列被归类为属于甲型冠状病毒。这些序列与在秘鲁的吸血蝙蝠中检测到的冠状病毒密切相关。对蝙蝠冠状病毒的持续监测可能有助于绘制和预测未来可能对人类健康产生重大影响的人畜共患病病原体。
The vampire bat (Desmodus rotundus) is a haematophagous animal that feeds exclusively on the blood of domestic mammals. Vampire bat feeding habits enable their contact with mammalian hosts and may enhance zoonotic spillover. Moreover, they may carry several pathogenic organisms, including coronaviruses (CoVs), for which they are important hosts. The human pathogens that cause severe acute respiratory syndrome (SARS-CoV), Middle East respiratory syndrome (MERS-CoV) and possibly coronavirus disease 2019 (SARS-CoV-2) all originated in bats but required bridge hosts to spread into human populations. To monitor the presence of potential zoonotic viruses in bats, the present work evaluated the presence of CoVs in vampire bats from southern Brazil. A total of 101 vampire bats were captured and euthanized between 2017 and 2019 in Rio Grande do Sul state, southern Brazil. The brain, heart, liver, lungs, kidneys and intestines were collected and macerated individually. The samples were pooled and submitted to high-throughput sequencing (HTS) using the Illumina MiSeq platform and subsequently individually screened using a pancoronavirus RT-PCR protocol. We detected CoV-related sequences in HTS, but only two (2/101; 1.98%) animals had CoV detected in the intestines by RT-PCR. Partial sequences of RdRp and spike genes were obtained in the same sample and the RdRp region in the other sample. The sequences were classified as belonging to Alphacoronavirus. The sequences were closely related to alphacoronaviruses detected in vampire bats from Peru. The continuous monitoring of bat CoVs may help to map and predict putative future zoonotic agents with great impacts on human health.