Lipopolysaccharide-induced expression of intercellular adhesion molecule-1 and chemokines in cultured human corneal fibroblasts
Lipopolysaccharide-induced expression of intercellular adhesion molecule-1 and chemokines in cultured human corneal fibroblasts
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DOI：
10.1167/iovs.04-0922
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发表时间：
2005-01-01
影响因子：
4.4
通讯作者：
Nishida, T
中科院分区：
文献类型：
--
作者：
Kumagai, N;Fukuda, K;Nishida, T
目的.细菌侵入角膜基质引起白细胞浸润和随后的角膜溃疡。通过检测革兰氏阴性菌的常见成分脂多糖（LPS）受体复合物的体外表达，以及LPS对培养的人角膜成纤维细胞中粘附分子表达和趋化因子释放的可能影响，研究角膜成纤维细胞在检测细菌侵入基质中的作用。通过逆转录-聚合酶链反应、酶联免疫吸附试验或免疫荧光分析检测LPS受体复合物、细胞间粘附分子（ICAM）-1和趋化因子白细胞介素（IL）-8和MCP-1的表达。发现角膜成纤维细胞含有编码Toll样受体-4、CD 14和MD-2的转录物，所有这些都是LPS受体复合物的组分。LPS可使角膜成纤维细胞表面ICAM-1表达增加，细胞内ICAM-1 mRNA表达量增加。类似地，LPS增加角膜成纤维细胞释放IL-8和MCP-1以及这些细胞中相应mRNA的量。低浓度人血清的存在可增强LPS的这些不同作用。角膜成纤维细胞可能通过识别LPS的存在并随后表达促进白细胞浸润的粘附分子和趋化因子而在角膜的防御系统中发挥重要作用。
PURPOSE. Invasion of bacteria into the corneal stroma induces the infiltration of leukocytes and subsequent corneal ulceration. The role of corneal fibroblasts in the detection of bacterial invasion into the stroma was investigated by examining the in vitro expression of the receptor complex for lipopolysaccharide (LPS), a common component of Gram-negative bacteria, as well as the possible effects of LPS on both the expression of adhesion molecules and the release of chemokines in cultured human corneal fibroblasts.METHODS. Expression of the LPS receptor complex, intercellular adhesion molecule (ICAM)-1, and the chemokines interleukin (IL)-8 and monocyte chemotactic protein (MCP)-1 was examined by reverse transcription - polymerase chain reaction, enzyme-linked immunosorbent assay, or immunofluorescence analysis.RESULTS. Corneal fibroblasts were found to contain transcripts encoding toll-like receptor-4, CD14, and MD-2, all of which are components of the LPS receptor complex. The expression of ICAM-1 at the surface of corneal fibroblasts and the amount of ICAM-1 mRNA in the cells were both increased by LPS. Similarly, LPS increased both the release of IL-8 and MCP-1 by corneal fibroblasts as well as the amounts of the corresponding mRNAs in these cells. These various effects of LPS were potentiated by the presence of a low concentration of human serum.CONCLUSIONS. Corneal fibroblasts may play an important role in the defense system of the cornea by recognizing the presence of LPS and subsequently expressing adhesion molecules and chemokines that promote leukocyte infiltration.