Evidence that repair and expression of potentially lethal damage cause the variations in cell survival after X irradiation observed through the cell cycle in Ehrlich ascites tumor cells.
Evidence that repair and expression of potentially lethal damage cause the variations in cell survival after X irradiation observed through the cell cycle in Ehrlich ascites tumor cells.
复制标题
通过艾利希腹水肿瘤细胞的细胞周期观察到的证据表明，潜在致命损伤的修复和表达会导致 X 照射后细胞存活率的变化。
作者：
G. Iliakis;M. Nüsse
测定了同步生长的艾氏腹水癌细胞(EAT细胞)经X射线照射后细胞周期不同阶段的存活率。照射后的S期细胞和G2+M期细胞表现出较高的杀伤活性，而照射后的G1期和S期中期细胞则表现出较强的抗性。这些变化是由于细胞在细胞周期中存活曲线肩宽(Drm Q)的变化，而平均致死剂量(D0)基本不变(0.8±0.05Gy.)。当诺康唑进一步增强细胞群体同步性时，得到S期初和有丝分裂时的指数存活曲线，D0=0.8Gy.当细胞(所有阶段)在平衡盐溶液中孵育6h后，潜在致命性损伤(PLD)被修复，导致$D{RmQ}$增加，而D0保持不变。照射后细胞用咖啡因(2-6 mM)或β-阿拉伯呋喃...
The survival of synchronously growing Ehrlich ascites tumor cells (EAT cells) was measured after X irradiation in various stages of the cell cycle. Cells at the beginning of S or in G2 + M phase showed a high level of killing, whereas cells irradiated in G1 or in the middle of S phase were more resistant. These changes resulted from a change in the survival curve shoulder width $(D_{{\rm q}})$ as cells passed through the cell cycle, and the mean lethal dose (D0) remained practically unchanged (0.8 ± 0.05 Gy). When synchronization of the cell population was further sharpened using nocodazole, exponential survival curves were obtained at the beginning of S phase and at mitosis with a $D_{0}=0.8$ Gy. When cells (in all stages) were incubated in balanced salt solution for 6 hr after irradiation, repair of potentially lethal damage (PLD) was observed, resulting in an increase in $D_{{\rm q}}$, while D0 remained constant. Treatment of the cells after irradiation with either caffeine (2-6 mM) or β-arabinofuranos...
辐射引起的分裂延迟的目标。
影响因子：
3.4
作者：
Schneiderman,MH;Hofer,KG
通讯作者：
Hofer,KG