Distinguishing Glycyrrhiza species using NMR-based Metabolomics
Distinguishing Glycyrrhiza species using NMR-based Metabolomics
复制标题
DOI：
10.1177/1934578x1801300122
复制
发表时间：
2018
影响因子：
1.8
通讯作者：
R. Suzuki;Fusako Nakano;H. Ohno;T. Murakami;Y. Okada;Y. Shirataki
中科院分区：
文献类型：
--
作者：
R. Suzuki;Fusako Nakano;H. Ohno;T. Murakami;Y. Okada;Y. Shirataki
甘草物种广泛用作天然药物和食品添加剂。因此，方法开发过程中的质量控制至关重要。在本研究中，我们进行了基于 NMR 的代谢组学分析来区分甘草物种（G. glabra、G. uralensis 和 G. infata）。甘草物种提取物的 1H-NMR 谱的主成分分析 (PCA) 能够对这三个物种进行分类。在 NMR 测量之前，我们对甘草根的甲醇提取物进行了固相萃取。大多数甘草物种的研究都使用质谱来区分物种。通过在 NMR 分析之前进行固相萃取，我们可以通过 PCA 评分图清楚地区分物种。这些结果表明，固相萃取能够在 NMR 代谢组学分析中清楚地区分甘草物种。此外，还使用核磁共振代谢组学根据物种对水提取物中固相萃取制备的皂苷级分进行了区分。甘草甜素等皂苷是甘草属植物的重要成分。因此，这些结果表明甘草中根皂苷存在物种特异性差异。
Glycyrrhiza species are widely used as natural medicines and as well as food additives. Quality control during methods development is therefore critically important. In this study, we conducted NMR-based metabolomics analyses to distinguish Glycyrrhiza species (G. glabra, G. uralensis, and G. inflata). Principle component analysis (PCA) of 1H-NMR spectra of extracts of Glycyrrhiza species enabled categorization of the three species. We conducted solid-phase extraction of methanol extracts of Glycyrrhiza roots prior to NMR measurements. Most studies of Glycyrrhiza species have used mass spectra to distinguish species. By conducting solid-phase extraction prior to NMR analysis, we could distinguish species clearly by PCA score plots. These results indicate that solid-phase extraction enables clear discrimination of Glycyrrhiza species in NMR metabolomics analyses. Furthermore, saponin fractions prepared by solid-phase extraction from water extracts were also discriminated according to the species using NMR metabolomics. Saponins such as glycyrrhizin are important constituents of Glycyrrhiza species. These results thus indicate that there are species-specific differences in root saponins in Glycyrrhiza.