One‐Step Enzymatic Purification of PCR Products for Direct Sequencing
One‐Step Enzymatic Purification of PCR Products for Direct Sequencing
复制标题
PCR 产物的一步酶纯化用于直接测序
DOI：
10.1002/0471142905.hg1106s30
复制
发表时间：
2001
影响因子：
--
通讯作者：
S. Blackshaw
中科院分区：
文献类型：
--
作者：
Jae Bum Kim;S. Blackshaw
聚合酶链式反应(PCR)已经成为分析DNA的一种强大而广泛的方法。该方法提供了方便的用于直接测序的PCR产物的酶纯化，而不需要进一步的亚克隆、沉淀或柱纯化。所有反应均可在96孔PCR板中进行，并与大规模测序兼容。该方法具有方便、快捷的特点。它适用于多种聚合酶链式反应模板，包括质粒模板、波长模板和基因组DNA模板。
The polymerase chain reaction (PCR) has become a powerful and widespread method for analyzing DNA. This protocol offers convenient enzymatic purification of PCR products for direct sequencing without the need for further subcloning, precipitation, or column purification. All reactions may be performed in 96‐well PCR plates and are compatible with large‐scale sequencing. This method is designed to be convenient and rapid. It is suitable for a variety of PCR templates, including plasmid, lambda, and genomic DNA.