Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA
Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA
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DOI：
10.1016/s0020-7519(99)00146-0
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发表时间：
1999-11-01
影响因子：
4
通讯作者：
Suzuki, N
中科院分区：
文献类型：
--
作者：
Tanaka, T;Xuan, XN;Suzuki, N
本研究将编码马巴伯虫裂殖子抗原2的基因插入杆状病毒转移载体中，获得表达B的重组病毒。分离马裂殖子抗原-2。两个B。用抗B的鼠抗血清检测马裂殖子抗原-2相关的重组杆状病毒表达的25和30 kDa肽。马裂殖子抗原-2;这些对应于天然B。马裂殖子抗原-2并分泌到昆虫细胞培养物的上清液中。重组B。马裂殖子抗原-2不受衣霉素处理的影响，表明B.马裂殖子抗原-2未被糖基化。重组B.通过测量对B的抗体应答来评价用于ELISA的马裂殖子抗原-2。马裂殖子抗原-2在实验感染B的马中的表达。等。(C)1999年澳大利亚寄生虫学会由Elsevier Science Ltd.出版，版权所有。
In this study, the gene encoding Babesia equi merozoite antigen-2 was inserted into a baculovirus transfer vector, and a recombinant virus expressing B. equi merozoite antigen-2 was isolated. Two B. equi merozoite antigen-2-related recombinant baculovirus-expressed peptides of 25 and 30 kDa were detected with a murine anti serum against B. equi merozoite antigen-2; these corresponded to the native B. equi merozoite antigen-2 and were secreted into the supernatants of insect cell cultures. Recombinant B. equi merozoite antigen-2 was not effected by tunicamycin treatment, indicating that B. equi merozoite antigen-2 was not glycosylated. The potential of recombinant B. equi merozoite antigen-2 for use in the ELISA was evaluated by measuring the antibody response to B. equi merozoite antigen-2 in horses experimentally infected with B. equi. (C) 1999 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.