Investigation of ospC Expression Variation among Borrelia burgdorferi Strains.
Investigation of ospC Expression Variation among Borrelia burgdorferi Strains.
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DOI：
10.3389/fcimb.2017.00131
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发表时间：
2017
影响因子：
5.7
通讯作者：
Lou Y
中科院分区：
文献类型：
--
作者：
Xiang X;Yang Y;Du J;Lin T;Chen T;Yang XF;Lou Y
外表面蛋白 C (OspC) 是伯氏疏螺旋体（莱姆病的病原体）研究最多的主要毒力因子。在体外培养时，伯氏疏螺旋体菌株之间的 OspC 水平差异很大，但人们对导致这种差异的原因知之甚少。有人提出，菌株间内源质粒含量的差异导致了OspC表型的变异，因为伯氏疏螺旋体含有超过21个内源线性(lp)和环状质粒(cp)，其中一些容易丢失。在这项研究中，我们分析了从伯氏疏螺旋体菌株 297 分离的几个克隆，该菌株是研究 ospC 表达最常用的菌株之一。通过利用最近公布的菌株297的质粒序列，我们开发了一种专门用于快速对伯氏疏螺旋体菌株297进行质粒分析的多重PCR方法。我们发现一些被认为具有完整质粒谱的常用297克隆实际上缺乏一些内源质粒。重要的是，结果表明质粒谱的差异不会导致克隆之间的 ospC 表达变异。此外，我们发现伯氏疏螺旋体克隆表达不同水平的BosR，这反过来导致不同水平的RpoS，并随后导致伯氏疏螺旋体菌株之间OspC水平的变化。
Outer surface protein C (OspC) is the most studied major virulence factor of Borrelia burgdorferi, the causative agent of Lyme disease. The level of OspC varies dramatically among B. burgdorferi strains when cultured in vitro, but little is known about what causes such variation. It has been proposed that the difference in endogenous plasmid contents among strains contribute to variation in OspC phenotype, as B. burgdorferi contains more than 21 endogenous linear (lp) and circular plasmids (cp), and some of which are prone to be lost. In this study, we analyzed several clones isolated from B. burgdorferi strain 297, one of the most commonly used strains for studying ospC expression. By taking advantage of recently published plasmid sequence of strain 297, we developed a multiplex PCR method specifically for rapid plasmid profiling of B. burgdorferi strain 297. We found that some commonly used 297 clones that were thought having a complete plasmid profile, actually lacked some endogenous plasmids. Importantly, the result showed that the difference in plasmid profiles did not contribute to the ospC expression variation among the clones. Furthermore, we found that B. burgdorferi clones expressed different levels of BosR, which in turn led to different levels of RpoS and subsequently, resulted in OspC level variation among B. burgdorferi strains.