PRODUCTION OF INACTIVE PHAGE P22 PARTICLES FOLLOWING INDUCTION
PRODUCTION OF INACTIVE PHAGE P22 PARTICLES FOLLOWING INDUCTION
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DOI：
10.1016/0042-6822(67)90150-x
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发表时间：
1967-01-01
期刊：
影响因子：
3.7
通讯作者：
ISRAEL, V
中科院分区：
文献类型：
--
作者：
ISRAEL, V
使用尾部附着的体外系统，证明在紫外线 (UV) 诱导鼠伤寒沙门氏菌 LT2 (P22) 后，形成的大多数颗粒缺乏功能性尾部。在所有研究温度下都发现了这种效应，但在 37 度及以上的温度下更为明显。热感应和自发感应也得到了类似的结果。只有在 42 度及以上的温度下，感染后才会发现无尾颗粒。对紫外线诱导的溶原菌的温度脉冲实验表明，在生长周期后期尾部合成受到高温的抑制。还有一个早期的温度敏感步骤，这可能会导致在 40°C 时产生不活跃的磁头。
Using an in vitro system for tail attachment, it is demonstrated that following ultraviolet (UV) induction of Salmonella typhlmurium LT2 (P22) most of the particles formed lack a functional tail. This effect is found at all temperatures studied, but is more pronounced at temperatures of 37[degree] and above. Similar results are described for thermal and spontaneous induction. Tailless particles are found following infection only at temperatures of 42[degree] and above. Temperature pulse experiments on UV-induced lysogens suggest that late in the growth cycle tail synthesis is inhibited by high temperature. There is also an earlier temperature-sensitive step, which probably causes the production of inactive heads at 40[degree].