Sequence of bacteriophage T3 DNA from gene 2.5 through gene 9.
Sequence of bacteriophage T3 DNA from gene 2.5 through gene 9.
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从基因 2.5 到基因 9 的噬菌体 T3 DNA 序列。
DOI：
10.1016/0022-2836(89)90102-2
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发表时间：
1989
影响因子：
5.6
通讯作者：
Molineux,IJ
中科院分区：
文献类型：
--
作者：
Beck,PJ;Gonzalez,S;Ward,CL;Molineux,IJ
已确定了噬菌体T3 DNA从基因2.5到基因9的核苷酸序列。除了调控位点外，该序列还预测了19个紧密排列的基因加上两个重叠的基因，在不同的阅读框中，另一个基因。这些基因大部分与噬菌体T7相应区域的基因高度同源。然而，有一些基因存在于一个噬菌体中，而不存在于另一个噬菌体中。这些明显的缺失几乎完全是基因大小，因此基因的紧密排列在T3和T7中保持相同。T3和T7 DNA之间不同程度的同源性，首先由Davis和Hyman在他们对DNA异双链的研究中注意到，这里也通过T3和T7核苷酸序列的比较来证明。许多同源性极高的区域直接与没有明显同源性的序列相连。这些数据表明，在它们的共同进化过程中，噬菌体T3和T7彼此重组，并与T7样噬菌体池中的其他成员重组。
The nucleotide sequence of bacteriophage T3 DNA, from gene 2·5 through gene 9 has been determined. In addition to regulatory sites, the sequence predicts 19 close-packed genes plus two genes that overlap, in a different reading frame, another gene. The majority of these genes are highly homologous to those in the corresponding region of bacteriophage T7. However, there are some genes that are present in one, but not the other, phage. These apparent deletions are almost exactly gene size and thus the close-packed organization of genes remains the same in T3 as in T7. The varying levels of homology between T3 and T7 DNAs, first noted by Davis and Hyman in their study of DNA heteroduplexes, are also demonstrated here by a comparison of T3 and T7 nucleotide sequences. Many regions of extremely high homology immediately abut sequences that have no apparent homology. These data suggest that bacteriophages T3 and T7 have recombined, both with each other and with other members of a pool of T7-like phages, during their co-evolution.