Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains.
Host Range-Associated Clustering Based on Multilocus Variable-Number Tandem-Repeat Analysis, Phylotypes, and Virulence Genes of Atypical Enteropathogenic Escherichia coli Strains.
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基于非典型肠致病性大肠杆菌菌株的多位点可变数量串联重复分析、系统发育型和毒力基因的宿主范围相关聚类。
DOI：
10.1128/aem.02796-18
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Nishikawa Y.
中科院分区：
文献类型：
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作者：
Parvej MS;Nakamura H;Alam MA;Wang L;Zhang S;Emura K;Kage-Nakadai E;Wada T;Hara-Kudo Y;Nishikawa Y.
采用分子流行病学方法对从649份家禽粪便中分离的36株日本和50株孟加拉国非典型致病性大肠杆菌（aEPEC）进行了分析。Clermont系统发育分型结果显示，A群的流行率（58%，50/86）高于B1群（31%，27/86）。β1型内膜蛋白在人类乳腺癌患者中普遍存在，在B1组（81%，22/27）和A组（70%，35/50）中均占优势。B1-β1菌株中约95%属于毒力组Ⅰ，其中日本株占77%; A-β1菌株中约17%（6/35）属于毒力组Ⅰ。多位点可变数目串联重复序列分析（MLVA）将菌株分布到52个不同的谱中，Simpson多样性指数（D）为73%。当数据与来自不同来源的142个先前菌株的数据相结合时，最小生成树形成了猪菌株、家禽菌株（不包括B1-β1）、来自健康人的菌株、牛和人类患者菌株以及B1-β1家禽菌株的5个区域。对萘啶酸的耐药性最常见（74%）。其中68%对≥3种抗菌药物耐药，其中大多数（91%）来自孟加拉国。通过系统聚类将菌株分为两组。相关矩阵分析表明，毒力基因与耐药性呈负相关。本研究表明，家禽，特别是日本家禽，可能是aEPEC的另一个储存库（致病菌群B1、毒力群I和β1型紧密粘附素）;然而，家禽菌株似乎与更接近牛菌株的患者菌株不同。非典型肠致病性大肠杆菌（aEPEC）是大肠杆菌中的一种致病性大肠杆菌。大肠杆菌，因为它具有用于在上皮上附着和消失的紧密蛋白基因（eae）。由于aEPEC即使在发达国家也普遍存在，我们以前使用分子流行病学方法来区分aEPEC作为人类病原体。本研究评估了家禽作为人类致疟性aEPEC的另一个来源。家禽可能是在日本患者菌株中发现的aEPEC（致病菌群B1、毒力群I和β1型粘附素）的来源。然而，最小生成树（MST）表明，与牛和患者菌株之间的距离相比，来自日本家禽的菌株与日本患者菌株之间的距离较远。孟加拉国的禽流感病毒株似乎不那么容易致病，但作为耐药性基因的来源是危险的。
Atypical enteropathogenic Escherichia coli (aEPEC) strains (36 Japanese and 50 Bangladeshi) obtained from 649 poultry fecal samples were analyzed by molecular epidemiological methods. Clermont’s phylogenetic typing showed that group A was more prevalent (58%, 50/86) than B1 (31%, 27/86). Intimin type β1, which is prevalent among human diarrheal patients, was predominant in both phylogroups B1 (81%, 22/27) and A (70%, 35/50). However, about 95% of B1-β1 strains belonged to virulence group I, and 77% of them were Japanese strains, while 17% (6/35) of A-β1 strains did. Multilocus variable-number tandem-repeat analysis (MLVA) distributed the strains into 52 distinct profiles, with Simpson’s index of diversity (D) at 73%. When the data were combined with those of 142 previous strains from different sources, the minimum spanning tree formed five zones for porcine strains, poultry strains (excluding B1-β1), strains from healthy humans, bovine and human patient strains, and the B1-β1 poultry strains. Antimicrobial resistance to nalidixic acid was most common (74%) among the isolates. Sixty-eight percent of them demonstrated resistance to ≥3 antimicrobial agents, and most of them (91%) were from Bangladesh. The strains were assigned into two groups by hierarchical clustering. Correlation matrix analysis revealed that the virulence genes were negatively associated with antimicrobial resistance. The present study suggested that poultry, particularly Japanese poultry, could be another reservoir of aEPEC (phylogroup B1, virulence group I, and intimin type β1); however, poultry strains seem to be apart from patient strains that were closer to bovine strains. Bangladeshi aEPEC may be less virulent for humans but more resistant to antibiotics.IMPORTANCEAtypical enteropathogenic Escherichia coli (aEPEC) is a diarrheagenic type of E. coli, as it possesses the intimin gene (eae) for attachment and effacement on epithelium. Since aEPEC is ubiquitous even in developed countries, we previously used molecular epidemiological methods to discriminate aEPEC as a human pathogen. The present study assessed poultry as another source of human diarrheagenic aEPEC. Poultry could be the source of aEPEC (phylogroup B1, virulence group I, and intimin type β1) found among patient strains in Japan. However, the minimum spanning tree (MST) suggested that the strains from Japanese poultry were far from Japanese patient strains compared with the distance between bovine and patient strains. Bangladeshi avian strains seemed to be less diarrheagenic but are hazardous as a source of drug resistance genes.