Blood group a antigen expression in platelets is prominently associated with glycoprotein Ib and IIb. Evidence for an A(1)/A(2) difference
Blood group a antigen expression in platelets is prominently associated with glycoprotein Ib and IIb. Evidence for an A(1)/A(2) difference
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DOI：
10.1046/j.1365-3148.1996.d01-52.x
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发表时间：
1996-03-01
影响因子：
1.5
通讯作者：
Wadenvik, H
中科院分区：
文献类型：
--
作者：
Hou, M;Stockelberg, D;Wadenvik, H
血型ABH抗原与血小板作为内在决定因子和外部吸附抗原相关，存在于鞘糖脂和糖蛋白（GPs）上。我们现在提供的直接证据表明，血型ABH抗原与血小板GPIb和GPIIb显著相关。通过免疫沉淀，小鼠单克隆抗a抗体沉淀表面生物素标记的血型a(1)血小板膜蛋白，其电泳特征与GPTb/IX和GPIIb/IIIa相同。通过SDS-PAGE免疫印迹分离出血型Al血小板蛋白，单克隆抗a抗体结合的蛋白具有与GPIb和GPIIb相同的电泳特征。当免疫亲和纯化的GPIb/IX和GPIIb/IIIa来源于血型O， Al-1和A(2)血小板，用于免疫印迹时，GPIb和GPIIb仅来自A(1)血小板结合单克隆抗A抗体。通过ELISA，使用GPIb （AP1）和GPIIb/IIIa复合物（AP2）特异性单克隆抗体捕获和保存血小板裂解液中的抗原，人抗A抗体与这些来自A(1)血型血小板的蛋白发生反应；A(2)型、O型和B型血小板蛋白无反应性。这些结果表明，血型A抗原与GPIb和GPIIb来源于血型A(1)而不是A(2)血小板相关。
Blood group ABH antigens are associated with platelets as intrinsic determinants and extrinsically adsorbed antigens, and exist both on glycosphingolipids and on glycoproteins (GPs). We now provide direct evidence that the blood group ABH antigens are prominently associated with platelet GPIb and GPIIb. By immunoprecipitation, a murine monoclonal anti-A antibody precipitated surface-biotin-labelled blood group A(1) platelet membrane proteins with electrophoretic characteristics identical to those of GPTb/IX and GPIIb/IIIa. By immunoblotting of SDS-PAGE separated blood group Al platelet proteins the monoclonal anti-A antibody bound to proteins with electrophoretic characteristics identical to those of GPIb and GPIIb. When immunoaffinity purified GPIb/IX and GPIIb/IIIa, derived from blood group O, Al-1 and A(2) platelets, were employed for immunoblotting, GPIb and GPIIb only from A(1) platelets bound the monoclonal anti-A antibody. By ELISA, wherein monoclonal antibodies specific for GPIb (AP1) and the GPIIb/IIIa complex (AP2) were used to capture and hold antigens from platelet lysate, human anti-A antibodies reacted with these proteins derived from blood group A(1) platelets; proteins from blood group A(2), O and B platelets showed no reactivity. These results indicate that blood group A antigen is associated with GPIb and GPIIb derived from blood group A(1) but not A(2) platelets.