NUCLEOTIDE-SEQUENCE AND STRUCTURE OF THE KLEBSIELLA-PNEUMONIAE PQQ OPERON
NUCLEOTIDE-SEQUENCE AND STRUCTURE OF THE KLEBSIELLA-PNEUMONIAE PQQ OPERON
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DOI：
10.1007/bf00280008
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发表时间：
1992-03-01
期刊：
影响因子：
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通讯作者：
POSTMA, PW
中科院分区：
文献类型：
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作者：
MEULENBERG, JJM;SELLINK, E;POSTMA, PW
对6940 bp klebsiella肺炎染色体DNA片段，其中包含涉及吡咯烷酚喹酮（PQQ）生物合成的基因。在PQQ操纵子中鉴定了六个开放式阅读框，PQQA，PQQB，PQQC，PQQD，PQQD，PQQE和PQQF，该镜头编码为2764（23氨基酸），33 464，28 986，28 986，10 436，42 881，42 881和83 616 DA DA DA DA DA（23 464，23 464，23 464，23 464，23 464，23 464，23 464） ， 分别。转录起点是通过引物扩展分析，PQQA上游映射的，并且可以识别启动子盒。在超长细胞中检测到PQQB，PQQC，PQQE和PQQF的基因产物，蛋白质的分子量与从核苷酸序列中推导的分子量相对应。 The gene products of pqqA, pqqB, pqqC, pqqD and pqqE show 49%-64% identity in amino acid sequence with those of pqqIV, pqqV, pqqI, pqqII and pqqIII respectively in the cloned pqq cluster of Acinetobacter calcoaceticus.由PQQF编码的84 kDa蛋白，该蛋白不存在于曲酸烟草的克隆PQQ簇中，但对于K. pneumoniae和Escherichia Coli中的PQQ生物合成至关重要，似乎属于蛋白酶家族。
A 6940 bp Klebsiella pneumoniae chromosomal DNA fragment, containing genes involved in pyrroloquinoline quinone (PQQ) biosynthesis, was sequenced. Six open reading frames, pqqA, pqqB, pqqC, pqqD, pqqE and pqqF were identified in the pqq operon, which coded for polypeptides of 2764 (23 amino acids), 33 464, 28 986, 10 436, 42 881 and 83 616 Da, respectively. The transcription startpoint was mapped by primer extension analysis, upstream of pqqA, and promoter boxes could be identified. The gene products of pqqB, pqqC, pqqE and pqqF were detected in maxi-cells and the molecular weights of the proteins corresponded with the molecular weights deduced from the nucleotide sequence. The gene products of pqqA, pqqB, pqqC, pqqD and pqqE show 49%-64% identity in amino acid sequence with those of pqqIV, pqqV, pqqI, pqqII and pqqIII respectively in the cloned pqq cluster of Acinetobacter calcoaceticus. The 84 kDa protein encoded by pqqF, which is not present in the cloned pqq cluster of A. calcoaceticus but which is essential for PQQ biosynthesis in K. pneumoniae and Escherichia coli, seems to belong to a family of proteases.