Neural conversion of ES cells by an inductive activity on human amniotic membrane matrix
Neural conversion of ES cells by an inductive activity on human amniotic membrane matrix
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DOI：
10.1073/pnas.0600104103
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发表时间：
2006-06-20
影响因子：
11.1
通讯作者：
Sasai, Yoshiki
中科院分区：
文献类型：
--
作者：
Ueno, Morio;Matsumura, Michiru;Sasai, Yoshiki
在这里，我们报告了一种人源性材料，具有强大的诱导活性，选择性地将ES细胞转化为神经组织。当在无血清培养基中的人羊膜基质成分上培养时，小鼠和人ES细胞均有效地分化为神经前体细胞[基于羊膜基质的ES细胞分化（AIMED）]。AIMED诱导的神经组织具有与通过与小鼠PA 6基质细胞共培养诱导的神经组织相似的区域特征（脑干）[一种称为基质细胞衍生的诱导活性（SDIA）培养的常见方法]。与SDIA培养物一样，AIMED系统适用于体外产生各种CNS组织，包括多巴胺能神经元、运动神经元和视网膜色素上皮。与使用动物细胞的SDIA方法相反，AIMED培养使用来自容易获得的人体组织的非细胞诱导材料;因此，AIMED应该提供用于产生用于临床应用的各种神经组织的更合适和通用的系统。
Here we report a human-derived material with potent inductive activity that selectively converts ES cells into neural tissues. Both mouse and human ES cells efficiently differentiate into neural precursors when cultured on the matrix components of the human amniotic membrane in serum-free medium [amniotic membrane matrix-based ES cell differentiation (AIMED)]. AIMED-induced neural tissues have regional characteristics (brainstem) similar to those induced by coculture with mouse PA6 stromal cells [a common method called stromal cell-derived inducing activity (SDIA) culture]. Like the SDIA culture, the AIMED system is applicable to the in vitro generation of various CNS tissues, including dopaminergic neurons, motor neurons, and retinal pigment epithelium. In contrast to the SDIA method, which uses animal cells, the AIMED culture uses a noncellular inductive material derived from an easily available human tissue; therefore, AIMED should provide a more suitable and versatile system for generating a variety of neural tissues for clinical applications.