Genome-wide identification, phylogeny, and expression analysis of the SWEET gene family in tomato
Genome-wide identification, phylogeny, and expression analysis of the SWEET gene family in tomato
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DOI：
10.1016/j.gene.2015.07.055
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发表时间：
2015-12-01
期刊：
影响因子：
3.5
通讯作者：
Jiang, Jing
中科院分区：
文献类型：
--
作者：
Feng, Chao-Yang;Han, Jia-Xuan;Jiang, Jing
Sweet(糖最终将被输出转运蛋白)基因家族编码含有七个跨膜螺旋的膜嵌入型糖转运蛋白，其中含有两个MtN3和唾液结构域。甜味物质在植物的生长、发育和对环境刺激的反应等多种生物过程中发挥着重要作用。在这里，我们对番茄基因组进行了详尽的搜索，最终鉴定出29个甜味基因。我们详细分析了这些蛋白质编码基因的结构、保守结构域和系统发育关系。我们还分析了甜基因在不同组织、器官和发育阶段的转录水平，以获得有关其功能的信息。此外，我们还研究了甜味基因在外源糖和逆境胁迫(高温和低温)下的表达模式。一些家族成员表现出组织特异性表达，而另一些则更普遍地表达。获得了大量的胁迫响应候选基因。本研究结果为深入了解番茄甜味基因的特性提供了依据，为进一步研究甜味基因的功能奠定了基础。(C)2015爱思唯尔B.V.保留所有权利。
The SWEET (Sugars Will Eventually Be Exported Transporters) gene family encodes membrane-embedded sugar transporters containing seven transmembrane helices harboring two MtN3 and saliva domain. SWEETs play important roles in diverse biological processes, including plant growth, development and response to environmental stimuli. Here, we conducted an exhaustive search of the tomato genome, leading to the identification of 29 SWEET genes. We analyzed the structures, conserved domains, and phylogenetic relationships of these protein-coding genes in detail. We also analyzed the transcript levels of SWEET genes in various tissues, organs, and developmental stages to obtain information about their functions. Furthermore, we investigated the expression patterns of the SWEET genes in response to exogenous sugar and adverse environmental stress (high and low temperatures). Some family members exhibited tissue-specific expression, whereas others were more ubiquitously expressed. Numerous stress-responsive candidate genes were obtained. The results of this study provide insights into the characteristics of the SWEET genes in tomato and may serve as a basis for further functional studies of such genes. (C) 2015 Elsevier B.V. All rights reserved.