PROTEIN CONTAMINANTS OF SODIUM DODECYL-SULFATE POLYACRYLAMIDE GELS
PROTEIN CONTAMINANTS OF SODIUM DODECYL-SULFATE POLYACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(83)90714-5
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
OCHS, D
中科院分区：
文献类型：
--
作者：
OCHS, D
使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和高灵敏度的检测方法，如银染、体外碘化和免疫反应(Western Blotting)，出现了许多伪影和令人困惑的结果。对生物学许多领域的研究，从与DNA紧密结合的蛋白质到中间丝的常见抗原决定因素，可能已经受到这种混乱的影响。一些怀疑污染的人认为2-巯基乙醇是污染源，但这份报告中的数据显示，污染的蛋白质是皮肤蛋白，特别是54-57 kDa和65-68 kDa的角蛋白。
Many artifacts and confusing results have arisen following the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and highly sensitive detection methods such as silver staining, in vitro iodination and immunological reactions (Western blotting). Investigations into many areas of biology, from proteins tightly bound to DNA to common antigenic determinants of intermediate filaments, may have been affected by this confusion. Some who have suspected contamination believed 2-mercaptoethanol to be the source, but data in this report show that the contaminating proteins are skin proteins, especially keratins ranging from 54-57 kDa [kilodaltons] and 65-68 kDa.