Comprehensive analysis of circRNAs expression profiles in different periods of MDBK cells infected with bovine viral diarrhea virus
Comprehensive analysis of circRNAs expression profiles in different periods of MDBK cells infected with bovine viral diarrhea virus
复制标题
牛病毒性腹泻病毒感染MDBK细胞不同时期circRNA表达谱综合分析
DOI：
10.1016/j.rvsc.2019.05.005
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发表时间：
2019-08-01
影响因子：
2.4
通讯作者：
Hu, Shengwei
中科院分区：
文献类型：
--
作者：
Li, Cunyuan;Li, Xiaoyue;Hu, Shengwei
CircRNA在疾病的调控中起着重要的调节作用。然而，我们对circRNA在BVDV感染的宿主复杂保护和病理机制中的作用了解有限。通过对BVDV感染后宿主细胞中circRNA的转录组分析，可以了解circRNA在BVDV感染调控中的生物学功能。在此，我们通过使用RNA-seq技术从BVDV感染的MBDK细胞（感染后12 h、24 h和48 h）中鉴定了总计19，118个circRNA。我们使用RT-PCR和DNA测序确认了几种circRNA，并进行qRT-PCR分析以鉴定几种circRNA表达和circRNA对RNase R消化的抗性。GO（Gene Ontology）和KEGG（京都基因与基因组百科全书）富集分析表明，差异表达的circRNA的宿主基因参与调控细胞增殖、凋亡、周期和病毒感染相关的信号通路。这些结果表明，宿主细胞中的circRNA在BVDV感染后发挥广泛的调节作用，并为研究BVDV感染后宿主细胞中的circRNA生物学提供了有价值的资源。
CircRNAs play an important regulatory role in the regulation of disease. However, we have a limited understanding of the role of circRNAs in the host's complex protective and pathological mechanisms of BVDV infection. Transcriptome analysis of circRNAs in host cells after BVDV infection may allow us to understand the biological functions of circRNAs in the regulation of BVDV infection. Here, we identified a total of 19,118 circRNAs from the MBDK cells (at 12 h, 24 h, and 48 h post-infection) infected with BVDV by using RNA-seq technology. We confirmed several circRNAs using RT-PCR and DNA sequencing, and qRT-PCR analysis was performed to identify several circRNAs expression and circRNAs resistance to RNase R digestion. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed that the host genes of differentially expressed circRNAs were involved in the regulation of cell proliferation, apoptosis, cycle and viral infection related signaling pathways. These results indicate that circRNA in host cells plays a broad regulatory role after BVDV infection and provides a valuable resource for studying circRNA biology in host cells after BVDV infection.