Identification of Autophagy in the Pine Wood Nematode Bursaphelenchus xylophilus and the Molecular Characterization and Functional Analysis of Two Novel Autophagy-Related Genes, BxATG1 and BxATG8.
Identification of Autophagy in the Pine Wood Nematode Bursaphelenchus xylophilus and the Molecular Characterization and Functional Analysis of Two Novel Autophagy-Related Genes, BxATG1 and BxATG8.
复制标题
松材线虫松材线虫自噬的鉴定及两个新的自噬相关基因BxATG1和BxATG8的分子表征和功能分析
DOI：
10.3390/ijms17030279
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发表时间：
2016-03-03
影响因子：
5.6
通讯作者：
Xue Q
中科院分区：
文献类型：
--
作者：
Deng LN;Wu XQ;Ye JR;Xue Q
松材线虫（Bursaphelenchusxylophilus）是一种生活史复杂、在恶劣和多变的环境条件下生存能力强、危害严重的松材线虫病。这种能力可能与自噬有关，而自噬在B中仍然知之甚少。而没有自噬相关基因的特征。本研究采用透射电镜证实自噬在B中存在。木生植物首先从B中克隆了BxATG 1和BxATG 8的全长cDNA。利用生物信息学方法对BxATG 1和BxATG 8进行了鉴定。使用原位杂交（ISH）研究自噬标记物BxATG 8的表达模式。BxATG 8在食管腺细胞和皮下缝细胞中表达。我们测试了RNA干扰（RNAi）对BxATG 1和BxATG 8的影响。结果表明，BxATG 1和BxATG 8可能与线虫在真菌垫上的繁殖有关。本研究证实了BxATG 1和BxATG 8在B中的分子特征和功能。为自噬和B之间的关系提供了基础信息。木生植物
The pine wood nematode, Bursaphelenchus xylophilus, causes huge economic losses in pine forests, has a complex life cycle, and shows the remarkable ability to survive under unfavorable and changing environmental conditions. This ability may be related to autophagy, which is still poorly understood in B. xylophilus and no autophagy-related genes have been previously characterized. In this study, transmission electron microscopy was used to confirm that autophagy exists in B. xylophilus. The full-length cDNAs of BxATG1 and BxATG8 were first cloned from B. xylophilus, and BxATG1 and BxATG8 were characterized using bioinformatics methods. The expression pattern of the autophagy marker BxATG8 was investigated using in situ hybridization (ISH). BxATG8 was expressed in esophageal gland and hypodermal seam cells. We tested the effects of RNA interference (RNAi) on BxATG1 and BxATG8. The results revealed that BxATG1 and BxATG8 were likely associated with propagation of nematodes on fungal mats. This study confirmed the molecular characterization and functions of BxATG1 and BxATG8 in B. xylophilus and provided fundamental information between autophagy and B. xylophilus.