Virion swelling is not required for cotranslational disassembly of cowpea chlorotic mottle virus in vitro
Virion swelling is not required for cotranslational disassembly of cowpea chlorotic mottle virus in vitro
复制标题
体外豇豆褪绿斑驳病毒的共翻译分解不需要病毒颗粒膨胀
作者：
F. Albert;J. Fox;M. Young
豇豆褪绿斑驳病毒 (CCMV) 病毒粒子分解并允许病毒粒子 RNA 翻译的机制尚不清楚。先前的模型表明，需要病毒体膨胀来暴露病毒体 RNA，以便在称为共翻译拆卸的过程中进行翻译（M. Brisco、R. Hull 和 T. M. A. Wilson, Virology 148:210-217, 1986；J. W. Roenhorst、J. W. M. van Lent 和 B. J. M. Verduin, Virology 164:91-98， 1988； Roenhorst、J. M. Verduin 和 R. W. Goldbach，《病毒学》168：138-146，1989）。我们实验室先前的工作已鉴定出 CCMV 外壳蛋白中的点突变，其导致病毒颗粒具有改变的膨胀特性（J. Fox, F. G. Albert, J. Speir, and M. J. Young, Virology 227:229-233, 1997; J. M. Fox, X. Zhu, J. A. Speir, and M. J. Young, Virology 222:115-122, 1996）。野生型和突变型 CCMV 病毒粒子用于将病毒粒子膨胀与病毒粒子 RNA 在无细胞麦芽提取物中翻译的能力相关联。无法膨胀的突变病毒粒子（cpK42R）在体内与野生型病毒粒子一样具有传染性，并且翻译的衣壳病毒粒子RNA水平与体外野生型病毒粒子相似。能够在体外膨胀但不能分解的突变病毒粒子 (cpR26C) 是非传染性的，并且在体外衣壳病毒粒子 RNA 的翻译水平严重降低。这些研究表明，CCMV 的共翻译分解不需要病毒体膨胀。此外，结果表明，除了膨胀之外，病毒颗粒中还存在 pH 依赖性结构转变，导致 RNA 暴露在体外进行翻译。提出了另一种模型，表明 CCMV 的共翻译分解涉及通过病毒体五重轴呈现病毒体 RNA。
The mechanism by which virions of cowpea chlorotic mottle virus (CCMV) disassemble and allow for translation of the virion RNA is not well understood. Previous models have suggested that virion swelling is required to expose the virion RNA for translation in a process referred to as cotranslational disassembly (M. Brisco, R. Hull, and T. M. A. Wilson, Virology 148:210-217, 1986; J. W. Roenhorst, J. W. M. van Lent, and B. J. M. Verduin, Virology 164:91-98, 1988; J. W. Roenhorst, J. M. Verduin, and R. W. Goldbach, Virology 168:138-146, 1989). Previous work in our laboratory has identified point mutations in the CCMV coat protein which result in virions with altered swelling characteristics (J. Fox, F. G. Albert, J. Speir, and M. J. Young, Virology 227:229-233, 1997; J. M. Fox, X. Zhao, J. A. Speir, and M. J. Young, Virology 222:115-122, 1996). The wild-type and mutant CCMV virions were used to correlate virion swelling with the ability of virion RNA to be translated in a cell-free wheat germ extract. Mutant virions unable to swell (cpK42R) are as infectious as wild-type virions in vivo, and the levels of translated encapsidated virion RNA are similar to those of wild-type virions in vitro. Mutant virions capable of swelling but not of disassembling in vitro (cpR26C) are noninfectious and have severely reduced levels of translation of the encapsidated virion RNA in vitro. These studies suggest that virion swelling is not required for the cotranslational disassembly of CCMV. Additionally, the results indicate that there is a pH-dependent structural transition in the virion, other than swelling, that results in the RNA's being exposed for translation in vitro. An alternative model suggesting that cotranslational disassembly of CCMV involves presentation of the virion RNA through the virion fivefold axis is proposed.
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影响因子：
3.7
作者：
ZHAO, XX;FOX, JM;YOUNG, MJ
通讯作者：
YOUNG, MJ
影响因子：
4
作者：
Ahlquist, Paul
通讯作者：
Ahlquist, Paul
影响因子：
5.6
作者：
Munshi, S;Liljas, L;Johnson, JE
通讯作者：
Johnson, JE
通过冷冻电子显微镜和 X 射线晶体学建立的 T = 3 二十面体动物病毒的准等效性的功能意义。
DOI：
10.1016/s0969-2126(00)00029-0
发表时间：
1994
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Cheng,RH;Reddy,VS;Olson,NH;Fisher,AJ;Baker,TS;Johnson,JE
通讯作者：
Johnson,JE