A NEW TYPE OF PAPILLOMAVIRUS DNA, ITS PRESENCE IN GENITAL CANCER BIOPSIES AND IN CELL-LINES DERIVED FROM CERVICAL-CANCER
A NEW TYPE OF PAPILLOMAVIRUS DNA, ITS PRESENCE IN GENITAL CANCER BIOPSIES AND IN CELL-LINES DERIVED FROM CERVICAL-CANCER
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DOI：
10.1002/j.1460-2075.1984.tb01944.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
HAUSEN, HZ
中科院分区：
文献类型：
--
作者：
BOSHART, M;GISSMANN, L;HAUSEN, HZ
从宫颈癌活检组织中克隆了一种新的乳头瘤病毒类型的DNA。获得两个长度为7.8和6.9个酶的EcoRI克隆，后者含有900个碱基对的缺失。两个克隆的BamHI片段用于表征DNA。它代表了一种独特类型的乳头瘤病毒，这是由其大小、其仅在低严格条件下与其他乳头瘤病毒类型的DNA的交叉杂交、其基因组与人乳头瘤病毒6型（HPV 6）和HPV 16原型的共线性比对以及其偶尔作为寡聚附加体出现所确定的。该病毒暂时被命名为HPV 18。在严格条件下，在非洲和巴西的9/36例宫颈癌、德国的2/13例宫颈肿瘤和1/10例阴茎癌中检测到与HPV 18杂交的DNA。良性肿瘤（17例宫颈发育不良，29例生殖器疣），8例原位癌和15例正常宫颈组织活检均未检测到HPV 18 DNA。然而，在HeLa、KB和C4-1系的人类细胞中发现了HPV 18相关DNA，这些细胞都来自宫颈癌。在4例宫颈癌活检中研究了病毒DNA的状态。DNA可能整合到宿主细胞基因组中。一个肿瘤提供了头到尾串联重复序列的证据，其中一些持续作为环状附加体。
DNA of a new papillomavirus type was cloned from a cervical carcinoma biopsy. Two EcoRI clones of 7.8 and 6.9 kilobases in length were obtained, the latter contained a 900-base pair deletion. The BamHI fragments of both clones were used to characterize the DNA. It represents a distinct type of papillomavirus as determined by its size, its cross-hybridization with DNA of other papillomavirus types under conditions of low stringency only, the co-linear alignment of its genome with human papillomavirus type 6 (HPV 6) and HPV 16 prototypes and its occasional occurrence as oligomeric episomes. The virus was tentatively designated HPV 18. DNA hybridizing with HPV 18 under stringent conditions was detected in 9/36 cervical carcinomas from Africa and Brazil, in 2/13 cervical tumors from Germany and 1/10 penile carcinomas. Benign tumors (17 cervical dysplasias, 29 genital warts), 8 carcinomata in situ and 15 biopsies of normal cervical tissue were devoid of detectable HPV 18 DNA. HPV 18-related DNA was found, however, in human cells of the HeLa, KB and C4-1 lines, all derived from cervical cancer. The state of the viral DNA was investigated in 4 cervical cancer biopsies. The DNA might be integrated into the host cell genome. One tumor provided evidence for head to tail tandem repeats, some of which persisted as circular episomes.