Cloning and characterization of a receptor-like protein kinase gene associated with senescence
Cloning and characterization of a receptor-like protein kinase gene associated with senescence
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DOI：
10.1104/pp.124.3.1305
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发表时间：
2000-11-01
期刊：
影响因子：
7.4
通讯作者：
Gepstein, S
中科院分区：
文献类型：
--
作者：
Hajouj, T;Michelis, R;Gepstein, S
衰老相关基因在植物衰老过程中表达上调，许多基因参与衰老组织代谢相关酶的编码。利用差异显示技术，我们确定了一个SAG豆（Phascolus vulgaris）叶片，专门在衰老过程中表达，并被指定为衰老相关受体样激酶（SARK）。推导的SARK多肽由信号肽、胞外区富含亮氨酸的重复序列、单个跨膜结构域和特征性丝氨酸/苏氨酸蛋白激酶结构域组成。SARK的mRNA水平在叶绿素损失和叶绿素a/b结合蛋白mRNA下降之前升高。与完整植株的叶片相比，分离的成熟菜豆叶片衰老速度加快，表现出类似的SARK信息积累的时间模式。光和细胞分裂素，延迟叶片衰老的开始，也延迟SARI基因的表达;相反，黑暗和乙烯，加速衰老，提前的SARK转录的最初外观。SARK蛋白的积累表现出类似于其mRNA的时间模式。SARK可能在叶片衰老的调控中起作用。
Senescence-associated genes are up-regulated during plant senescence and many have been implicated in encoding enzymes involved in the metabolism of senescing tissues. Using the differential display technique, we identified a SAG in bean (Phascolus vulgaris) leaf that was exclusively expressed during senescence and was designated senescence-associated receptor-like kinase (SARK). The deduced SARK polypeptide consists of a signal peptide, a leucine-rich repeat in the extracellular region, a single membrane-spanning domain, and the characteristic serine/threonine protein kinase domain. The mRNA level for SARK increased prior to the loss of chlorophyll and the decrease of chlorophyll a/b-binding protein mRNA. Detached mature bean leaves, which senesce at an accelerated rate compared with leaves on intact plants, showed a similar temporal pattern of SARK message accumulation. Light and cytokinin, which delayed the initiation of leaf senescence, also delayed SARI; gene expression; in contrast, darkness and ethylene, which accelerated senescence, advanced the initial appearance of the SARK transcript. SARK protein accumulation exhibited a temporal pattern similar to that of its mRNA. A possible role for SARK in the regulation of leaf senescence was considered.