Partial characterization of a carotenoid droplet ATPase and its possible significance in carotenoid droplet dispersion in goldfish xanthophores.
Partial characterization of a carotenoid droplet ATPase and its possible significance in carotenoid droplet dispersion in goldfish xanthophores.
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类胡萝卜素液滴 ATP 酶的部分表征及其在金鱼黄色细胞中类胡萝卜素液滴分散中的可能意义。
DOI：
10.1002/cm.970150303
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发表时间：
1990
影响因子：
--
通讯作者：
Tchen,TT
中科院分区：
文献类型：
--
作者：
Wu,BY;Yu,FX;Lynch,TJ;Taylor,JD;Tchen,TT
类胡萝卜素液滴在渗透的金鱼黄色素中的分散依赖于三磷酸腺苷、F-肌动蛋白和胞浆。我们在这里报告，马达(ATPase，转运体)驻留在被渗透的细胞残留物中，而不是在胞浆中。我们还报告说，类胡萝卜素液滴具有不同于传统肌球蛋白、动力蛋白或离子泵的ATPase。它的活性似乎与类胡萝卜素液滴制剂中肌动蛋白的含量有关。类胡萝卜素液滴蛋白Mr 72,000(P72)被8-叠氮基-三磷酸腺苷辐射标记，伴随着类胡萝卜素液滴ATPase活性的丧失。我们推测该蛋白可能是导致类胡萝卜素液滴分散的ATPase。
The dispersion of carotenoid droplets in permeabilized goldfish xanthophores is dependent on ATP, F‐actin, and cytosol. We report here that the motor (ATPase, translocator) resides with the permeabilized cell remnants and not in the cytosol. We also report that the carotenoid droplets have an ATPase that is not conventional myosin, dynein, or an ion pump. Its activity appears to correlate with the actin content of the carotenoid droplet preparation. A carotenoid droplet protein of Mr72,000 (p72) is shown to be labeled by irradiation with 8‐azido‐ATP with concomitant loss of ATPase activity of the carotenoid droplets. We propose that this protein may be the ATPase responsible for carotenoid droplet dispersion.