The effect of dietary carbohydrate on genes for fatty acid synthase and inflammatory cytokines in adipose tissues from lean and obese subjects
The effect of dietary carbohydrate on genes for fatty acid synthase and inflammatory cytokines in adipose tissues from lean and obese subjects
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DOI：
10.1016/j.jnutbio.2007.02.013
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发表时间：
2008-04-01
影响因子：
5.6
通讯作者：
Hirsch, Jules
中科院分区：
文献类型：
--
作者：
Hudgins, Lisa C.;Baday, Aline;Hirsch, Jules
背景：富含单糖的低脂饮食可显著刺激肝脏新生脂肪生成（DNL）。然而，人体脂肪组织中控制DNL的关键酶脂肪酸合成酶（FAS）的饮食反应性尚不确定。假设：当高热量、低脂肪、高糖饮食导致肝脏DNL升高时，瘦肉和肥胖受试者的脂肪组织FAS mRNA增加。设计：通过随机顺序交叉设计，12名瘦人志愿者和7名肥胖志愿者被给予两种高热量饮食（10% vs 30%脂肪；75% vs 55%碳水化合物；糖/淀粉60/40），每两周。采用同位素和非同位素方法比较腹部和臀部脂肪组织中的FAS mRNA与血清中肝脏DNL的含量。还检测了脂肪组织中肿瘤坏死因子- α和IL-6的mRNA，它们是调节DNL的炎症细胞因子。结果：低脂高糖饮食可使大鼠肝脏最大DNL增加4倍
Background: Hepatic de novo lipogenesis (DNL) is markedly stimulated in humans by low-fat diets enriched in simple sugars. However, the dietary responsiveness of the key enzyme controlling DNL in human adipose tissue, fatty acid synthase (FAS), is uncertain.Hypothesis: Adipose tissue mRNA for FAS is increased in lean and obese subjects when hepatic DNL is elevated by a eucaloric, low-fat, high-sugar diet.Design: Twelve lean and seven obese volunteers were given two eucaloric diets (10% vs. 30% fat; 75% vs. 55% carbohydrate; sugar/starch 60/40) each for 2 weeks by a random-order cross-over design. FAS mRNA in abdominal and gluteal adipose tissues was compared to hepatic DNL measured in serum by isotopic and nonisotopic methods. Adipose tissue mRNA for tumor necrosis factor-alpha and IL-6, which are inflammatory cytokines that modulate DNL, was also assayed.Results: The low-fat high-sugar diet induced a 4-fold increase in maximum hepatic DNL (P