Comparative interactomics for virus-human proteinprotein interactions: DNA viruses versus RNA viruses
Comparative interactomics for virus-human proteinprotein interactions: DNA viruses versus RNA viruses
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DOI：
10.1002/2211-5463.12167
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发表时间：
2017-01-01
期刊：
影响因子：
2.6
通讯作者：
Ulgen, Kutlu O.
中科院分区：
文献类型：
--
作者：
Durmus, Saliha;Ulgen, Kutlu O.
病毒是专性细胞内病原体，完全依赖于其宿主生存和繁殖。病毒在感染过程中利用宿主细胞过程和逃避宿主免疫系统的策略可能因病毒遗传物质的类型而大不相同。只有通过更好地理解病原体-宿主相互作用（PHIs）才能更好地理解这些病毒感染机制，这些病原体-宿主相互作用使病毒能够进入宿主细胞并操纵细胞机制以使其自身受益。实验验证的蛋白质-蛋白质相互作用（PPI）的病原体-宿主系统的数据只是在过去的十年中才成为大规模。在这项研究中，我们比较分析了目前属于DNA和RNA病毒及其人类宿主的PHI网络，以了解这些病毒群体使用的感染策略。我们研究了人类蛋白质在PHI网络中的功能特性，观察和比较了DNA和RNA病毒的攻击策略。我们观察到DNA病毒能够在感染期间同时攻击人类细胞和代谢过程。另一方面，RNA病毒优先与在特定细胞过程中以及在细胞内转运和定位中起作用的人蛋白质相互作用。通过观察病毒靶向的人类蛋白质，我们提出核内异质核糖核蛋白和转运蛋白作为潜在的抗病毒治疗靶点。在病毒攻击人类蛋白质的策略方面，观察到的常见和特异性感染机制可能为进一步设计广泛和特异的下一代抗病毒治疗提供关键信息。
Viruses are obligatory intracellular pathogens and completely depend on their hosts for survival and reproduction. The strategies adopted by viruses to exploit host cell processes and to evade host immune systems during infections may differ largely with the type of the viral genetic material. An improved understanding of these viral infection mechanisms is only possible through a better understanding of the pathogen-host interactions (PHIs) that enable viruses to enter into the host cells and manipulate the cellular mechanisms to their own advantage. Experimentally-verified protein- protein interaction (PPI) data of pathogen-host systems only became available at large scale within the last decade. In this study, we comparatively analyzed the current PHI networks belonging to DNA and RNA viruses and their human host, to get insights into the infection strategies used by these viral groups. We investigated the functional properties of human proteins in the PHI networks, to observe and compare the attack strategies of DNA and RNA viruses. We observed that DNA viruses are able to attack both human cellular and metabolic processes simultaneously during infections. On the other hand, RNA viruses preferentially interact with human proteins functioning in specific cellular processes as well as in intracellular transport and localization within the cell. Observing virustargeted human proteins, we propose heterogeneous nuclear ribonucleoproteins and transporter proteins as potential antiviral therapeutic targets. The observed common and specific infection mechanisms in terms of viral strategies to attack human proteins may provide crucial information for further design of broad and specific next-generation antiviral therapeutics.