Systematics of Malaysian woolly bats (Vespertilionidae: Kerivoula) inferred from mitochondrial, nuclear, karyotypic, and morphological data
Systematics of Malaysian woolly bats (Vespertilionidae: Kerivoula) inferred from mitochondrial, nuclear, karyotypic, and morphological data
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DOI：
10.1644/09-mamm-a-361.1
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发表时间：
2010-10-01
影响因子：
1.7
通讯作者：
Baker, Robert J.
中科院分区：
文献类型：
--
作者：
Khan, Faisal Ali Anwarali;Solari, Sergio;Baker, Robert J.
使用来自多个独立来源的数据来检查物种边界是识别遗传上分离的进化单位的适当和可靠的方法。利用细胞色素h （Cytb）、细胞色素c氧化酶（COI）、扩增片段长度多态性（aflp）、核型和形态学5个数据集，对东南亚毛蝙蝠的系统发育关系和物种界限进行了估计。我们对来自马来西亚的54个Kerivoula标本进行了遗传分析，并将其划分为目前报告的马来西亚10个物种中的6个。核aflp（33个标本）和Cytb（51个标本）和COI（48个标本）的线粒体DNA序列的系统发育分析结果显示，统计上支持相似的物种水平分支，分支顺序变化最小。通过颅骨和牙齿形态与原始物种描述的比较，我们将得到的系统发育分支划分为K. hardwickii， K. intermedia， K. lenis， K. minuta， K. papillosa， K. pellucida和1个未知物种。核型进一步证明了6个进化支之间的差异。鉴定出5种不同的核型，其中2种核型难以区分。我们将COI基因序列数据与来自东南亚的110个Kerivoula标本进行了比较，这些标本由生命条形码数据库的研究人员提供。我们的Cytb和AFLP物种鉴定结果与COI数据库一致。在hardwickii和minuta中，Cytb和COI基因在种内的序列差异约为5-7%。放松分子钟分析表明，Kerivoulinae的起源时间为渐新世晚期至中新世早期，至少有6个种的种内多样化事件与上新世晚期和更新世时期相吻合。与马来西亚半岛和沙捞越（婆罗洲西南部）的样本相比，来自沙巴（婆罗洲东北部）的样本显示出相对较高的遗传差异，表明婆罗洲有更新世或上新世的避难所。我们的结论是，在婆罗洲至少有一个不同的Kerivoula谱系尚未被描述，并且某些物种的种内地理变异与先前关于婆罗洲动植物多样性的研究一致。DOI: 10.1644 / 09 - mamm - 361.1
Examining species boundaries using data from multiple independent sources is an appropriate and robust method to identify genetically isolated evolutionary units. We used 5 data sets cytochrome h (Cytb), cytochrome c oxidase (COI), amplified fragment length polymorphisms (AFLPs), karyotypes, and morphology to estimate phylogenetic relationships and species limits within woolly bats, genus Kerivoula, from Southeast Asia. We genetically analyzed 54 specimens of Kerivoula from Malaysia, assigned to 6 of the 10 species currently reported from the country. Phylogenetic analyses of nuclear AFLPs (33 specimens) and mitochondrial DNA sequences from Cytb (51 specimens) and COI (48 specimens) resulted in similar statistically supported species-level clades with minimal change in branching order. Using comparisons of cranial and dental morphology and original species descriptions, we assigned the resulting phylogenetic clades to K. hardwickii, K. intermedia, K. lenis, K. minuta, K. papillosa, K pellucida, and 1 unidentified species. Karyotypes further documented variability among the 6 clades. Five different karyotypes were identified, with 2 species having indistinguishable karyotypes. We compared our COI gene sequence data to 110 specimens of Kerivoula from Southeast Asia made available by researchers of the Barcode of Life Database. Our Cytb and AFLP species identifications were congruent with those in the COI database. Intraspecific geographic variation of about 5-7% sequence divergence was observed in Cytb and COI genes within both K. hardwickii and K. minuta. Relaxed molecular clock analyses indicated a late Oligocene to early Miocene origin of the Kerivoulinae with intraspecific diversification events coinciding with the late Pliocene and Pleistocene epochs in at least 6 species. Specimens from Sabah (northeastern Borneo) showed relatively high genetic divergence compared to those between Peninsular Malaysia and Sarawak (southwestern Borneo), indicating Pleistocene or Pliocene refugia in Borneo. We conclude that at least 1 distinct lineage of Kerivoula is yet to be described from Borneo and that the intraspecific geographic variation in some species agrees with previous studies on the diversification of flora and fauna in Borneo. DOI: 10.1644/09-MAMM-A-361.1